Czech

Not applicable.

Successful completion of the course the student will gain the following knowledge and skills:
1. Students will be familiar with the principles of functions of various types of mass spectrometers;
2. Students will be able to choose the optimal device for highly efficient solutions for specific analytical problems;
3. Students will know the basics of mass spectra interpretation, depending on the ionization technique used and the analyzer resolution.

Basic knowledge of physics - kinematics, the movement of charged particles in magnetic and electric field
Good knowledge of basic, inorganic, organic, physical and analytical chemistry

Not applicable.

The course uses teaching methods in form of Lecture - 2 teaching hours per week. The e-learning system (LMS Moodle) is available to teachers and students.

The exam consists of a written and oral examination. The subject of the written interpretation of the given mass spectrum, the oral examination is focused on the verification of student's knowledge in the range of themes discussed in the lectures. In the final assessment, the result of the written part contributes by 40%, the result of oral part by 60%.

1. Introduction to mass spectrometry, theoretical basics, natural isotopic distribution of elements and its importance in MS.
2. Instrumentation of mass spektrometry.
3. Ionization techniques in mass spektrometry.
4. Mass analyzers.
5. Hyphenated techniques (GC/MS, LC/MS, CE/MS).
6. Hyphenated techniques (MS/MS).
7. Mass spectrometry imaging.
8. Inorganic mass spectrometry.
9. Interpretation of mass spectra using electron ionization, fragmentation rules.
10. Interpretation of mass spectra – practicing.
11. Interpretation of mass spectra using soft ionization techniques.
12. Interpretation of mass spectra using soft ionization techniques – practicing.
13. Practical applications of mass spectrometry.

Not applicable.

The aim of this course is:
- to familiarize students with the theoretical foundations of mass spectrometry
- to provide an overview of current instrumentation of mass spectrometry
- to introduce students to the basic concepts interpretation of mass spectra

Attendance at lectures is optional and is not controlled, but it is recommended.

Not applicable.

Not applicable.

Douglas A. Skoog et.al.: Analytická chemie, 2019 (CS)
J. H. Gross: Mass Spectrometry, Third Edition, 2017 (EN)
K. Záruba a spol.: Analytická chemie 2. díl, 2016 (CS)

Not applicable.