Course detail

Biochemistry I

FCH-BAA_BCH1Acad. year: 2020/2021

Organization and composition of living cells and cellular compartments. Amino acids - composition and structure, peptides, proteins. Methods of isolation and separation of proteins. Functional roles of proteins. Nucleic acids. Carbohydrates. Lipids. Biomembranes. Metabolic pathways. termodynamic relationships and energy-rich components. Enzymology, enzyme catalysis. Enzyme kinetics, inhibition of enzymes. Regulation of enzyme activity. Nucleic acids. Applied biochemistry.

Language of instruction

English

Number of ECTS credits

4

Mode of study

Not applicable.

Offered to foreign students

Of all faculties

Learning outcomes of the course unit

After passing of the course Biochemistry I is student mastered of overview about cell composition, structure, properties and function of basic groups of biomolecules, e.g. amino acids, proteins, saccharides, lipids and nucleic acids. Further, basic knowledge about structure of biomemrbanes and types of membrane transport are introduced and basic principles of enzyme catalysis, kinetics and regulation of enzyme activity are elucidated. Next, overview about methods of isolation and characterization of biopolymers will be obtained and some applications including enzyme engineering and genetic engineering will be discussed.

Prerequisites

Knowledge of organic chemistry, basic knowledge of physical chemistry.

Co-requisites

Not applicable.

Planned learning activities and teaching methods

The course uses teaching methods in form of Lecture - 2 teaching hours per week. The e-learning system (LMS Moodle) is available to teachers and students.

Assesment methods and criteria linked to learning outcomes

Credit test. Examination - written and oral part.

Course curriculum

Organization and composition of living cells and cellular compartments.
Amino acids - composition and structure, peptides, proteins.
Methods of isolation, purification and separation of proteins.
Functional roles of proteins.
Carbohydrates - monosaccharides, polysaccharides, glycoproteins.
Lipids - structure and classification. Triglycerides, phospholipids. Isoprenoids.
Biomembranes. Membrane transport.
Enzymology, enzyme catalysis. Substrate specificity. Cofactors. Regulation of enzyme activity.
Enzyme kinetics, Michaelis-Menten equation. Inhibition of enzymes.
Metabolic pathways. termodynamic relationships and energy-rich components.
Nucleic acids - structure and function. DNA, RNA.
Applide biochemistry - modern biotechnology, genetic engineering.

Work placements

Not applicable.

Aims

The aim of the course Biochemistry I is to introduce cell composition and to characterize structure, properties and function of basic groups of biomolecules, e.g. amino acids, proteins, saccharides, lipids and nucleic acids. Further, structure of biomemrbanes and types of membrane transport are explained and basic principles of enzyme catalysis, kinetics and regulation of enzyme activity are elucidated. Next, methods of isolation and characterization of biopolymers are introduced and some applications including enzyme engineering and genetic engineering are discussed.

Specification of controlled education, way of implementation and compensation for absences

none

Recommended optional programme components

Not applicable.

Prerequisites and corequisites

Not applicable.

Basic literature

Metzler D.A.: Biochemistry. Academic Press, 2003. (EN)

Recommended reading

Alberts et al: Molecular Biology of the Cell. Garland Science, 2003. (EN)
Berg, Tymoczko, Stryer: Biochemistry. W.H.Freeman, 2002. (EN)

Elearning

Classification of course in study plans

Type of course unit

 

Lecture

26 hod., optionally

Teacher / Lecturer

Elearning