Course detail
Laboratory Classes in Special Toxicology
FCH-MCO_SPT_PAcad. year: 2012/2013
Practical course of special toxicology to increased knowledge of special toxicology and will allow connection of theoretical and practical knowledge. The scope of practical course is to introduce students with the possibilities of determination of some toxic substances in biotic and abiotic matrices and determination of selected toxicological data directly from an experiment in laboratory conditions. Students will gain an overview of selected experimental methods used in toxicology, preparation of abiotic and biotic matrices for the determination of selected toxic compounds. Students will be introduced to a screening technique TLS (1D, 2D), the instrumental technique used in toxicology (atomic absorption spectrometry - GF AAS, F AAS), methodology and evaluation of toxicity tests (test viability, cytotoxicity test). The analysis and evaluation of case studies from toxicology are included of practicum of special toxicology.
Language of instruction
Number of ECTS credits
Mode of study
Learning outcomes of the course unit
1st Students will gain an overview of selected experimental methods used in toxicology.
2d Students will be able to prepare of abiotic and biotic matrices for the determination of selected toxic compounds.
3rd Students will know a screening technique TLS (1D, 2D).
4th Students will know instrumental technique used in toxicology (atomic absorption spectrometry).
5th Students will know the methodology and evaluation of toxicity tests (Test with neutral red - test viability, MTT test - cytotoxicity tests, trypan blue exclusion test - a test of viability and cytotoxicity).
6th Students will be able will be able to evaluate the case studies.
Prerequisites
Co-requisites
Planned learning activities and teaching methods
Assesment methods and criteria linked to learning outcomes
Course curriculum
2nd Extraction procedures for the preparation of biotic and abiotic matrices
3rd Identification of the type of nicotine alkaloids in Nicotiana tabacum
4th Identification of biologically active compounds (derivatives Pyranocumarin)
6th Application of TLC of determine PAHs and separation compounds with using 2D TLC
7th Identification and quantification of toxic metals toxic metals (cadmium, arsenic) in abiotic and biotic matrices with using GF AAS
8th Identification and quantification of toxic metals toxic metals (chromium, lead) in biotic and abiotic matrices with using F AAS
9th Test with neutral red - test viability
10th MTT test - cytotoxicity tests
11th Test with trypane blue - test viability and cytotoxicity
12th Case Study of toxicology
13th Credit
Work placements
Aims
Specification of controlled education, way of implementation and compensation for absences
Recommended optional programme components
Prerequisites and corequisites
Basic literature
Recommended reading
Hrdina, V. a kol., Přírodní toxiny a jedy, Galén, Praha, 2004 (CS)
Classification of course in study plans
- Programme NPCP_CHTOZP Master's
branch NPCO_CHTOZP , 1 year of study, winter semester, compulsory
- Programme NKCP_CHTOZP Master's
branch NKCO_CHTOZP , 1 year of study, winter semester, compulsory
- Programme CKCP_CZV lifelong learning
branch CKCO_CZV , 1 year of study, winter semester, compulsory-optional
Type of course unit
Guided consultation in combined form of studies
Teacher / Lecturer
Laboratory exercise
Teacher / Lecturer