Course detail

Laboratory Classes in Instrumental and Structure Analysis - FSBT

FCH-MCA_ISA_PAcad. year: 2012/2013

Practical class to the lecture Instrumental and Structure Analysis.
Students are familiarized with instrumental analytical techniques used by control and analysis of foodstuff and food raw materials during this class. There are covered exercises of separation techniques (chromatography and electrophoresis), exercises of thermic analysis, elementar trace analysis, spectral, structural and mass analysis.

Language of instruction

Czech

Number of ECTS credits

Mode of study

Not applicable.

Guarantor

RNDr. Milena Vespalcová, Ph.D.

Department

Institute of Food Science and Biotechnology (ÚCHPBT)

Learning outcomes of the course unit

Information concerning basic instrumental analytical methods
Practical training of subjects from the course Instrumental and Structural Analysis

Prerequisites

Successful completion of the practical class is conditional on the basic knowledge of the lecture Instrumental and Structure Analysis.

Co-requisites

Not applicable.

Planned learning activities and teaching methods

Teaching methods depend on the type of course unit as specified in the article 7 of BUT Rules for Studies and Examinations.

Assesment methods and criteria linked to learning outcomes

Graded-course credit on the base of submitted protocols and semestral thesis.

Course curriculum

1. Atomic absorption spectrometry - AAS
2. Mass spectrometry with inductively coupled plasma - ICP-MS
3. Electromigration methods - CZE
4. Electromigration methods - gel electrophoresis
5. Electron paramagnetic resonance - EPR
6. Nuclear magnetic resonance
7. Liquid chromatography (HPLC)
8. Liquid chromatography with mass detection (LC_MS)
9. Gas chromatography (GC)
10. Gas chromatography with mass detection (GC-MS)
11. Spectrophotometry - UV-VIS
12. Fluorescence microscopy

Work placements

Not applicable.

Aims

The goal of the classt is to practice theoretical knowledge acquired on the lecture "Food Analysis" in a system of practical exercises.

Specification of controlled education, way of implementation and compensation for absences

The attendance is required. In case of absence, the particular seminar can be compensated by attending with another group or in the last week of semester. An unexcused absence is a reason for the loss of the course-unit credit.
The examination of basic knowledge relating to the particular project is done at the beginning of each exercise.
Laboratory reports are required till 7 days after the last exercise at the latest.

Recommended optional programme components

Not applicable.

Prerequisites and corequisites

Not applicable.

Basic literature

Not applicable.

Type of course unit

Guided consultation in combined form of studies

39 hod., optionally

Teacher / Lecturer

RNDr. Milena Vespalcová, Ph.D.

Laboratory exercise

39 hod., compulsory

Teacher / Lecturer

doc. Ing. Eva Vítová, Ph.D.
doc. Ing. Pavel Diviš, Ph.D.
prof. Ing. Josef Čáslavský, CSc.
Ing. Andrea Lichnová, Ph.D.
RNDr. Milena Vespalcová, Ph.D.
RNDr. Mária Veselá, Ph.D.
Ing. Jaromír Pořízka, Ph.D.
Ing. Petra Skoumalová, Ph.D.
Ing. Miloš Dvořák, Ph.D.
Ing. Andrea Němcová, Ph.D.
Ing. Jitka Cetkovská, Ph.D.