High resolution ultrasonic spectroscopy for measuring of interactions of hyaluronan with surfactants contribution

conference paper

English

Hyaluronan is natural biopolymer occurring in the human body and it is used as controlled drug delivery transport system, as a material for scaffold in tissue engineering, as a component of a bandage by wound healing processes, as an alternate fluid in human joints, as a space filling matter in plastic surgery and last but not least as a hydration matter in cosmetics. High resolution ultrasonic spectroscopy (HR-US) is a new technique for material analysis based on the measurements of parameters of ultrasonic waves propagating through samples. This technique is based on the measurements of velocity and attenuation on acoustical waves at high, ultrasonic frequencies propagating through materials. This technique allows direct and non-destructive measurements without formation of derivatives or changing state of sample. Advantage of this technique is the ability of ultrasonic waves to propagate through optically non-transparent materials. The analyzed sample can be highly coloured or even opaque. In this contribution, a high-resolution ultrasonic spectroscopy technology (HR US 102) has been employed to analyse critical aggregation concentration of the surfactant in water and in the solution with polyelectrolyte. Anionic polymer sodium hyaluronate (1 g/kg – HYA/water) and cationic surfactant hexadecyltrimethylammonium bromide (CTAB) were used for the experiment. Ultrasound velocity and attenuation at 7MHz were measured. Critical aggregation concentration of CTAB in water was determined as 1 mmol/kg. Micelles were formed above CMC in water and in the solution with hyaluronan below this concentration there were not any free surfactant and above this concentration micelles were on the chain of hyaluronan. These results were confirmed by measurement of ultrasound attenuation. Work is focuses on the interactions between cationic surfactants and hyaluronan in the different molecular weight and concentration measured by the method called high resolution ultrasonic spectroscopy. The experimentswas made in water and sodium chloride solution.

hyaluronan, surfactant, drug delivery, komplex CTAB-hyaluronan

KARGEROVÁ, A.; PEKAŘ, M.

2012

7. 12. 2012

Vysoké učení technické v Brně, Fakulta chemická, Purkyňova 464/118, 612 00, Brno

Brno

978-80-214-4425-6

Studentská konference Chemie je život, Sborník abstraktů

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