Publication detail

The Use of Real Time PCR Method for Evaluation of Magnetic Microspheres

TRACHTOVÁ, Š. RITTICH, B. HORÁK, D. ŠPANOVÁ, A.

Original Title

Type

abstract

Language

English

Original Abstract

The real-time quantitative PCR (qPCR) was used for the evaluation of non-porous HEMA based magnetic microspheres on the PCR course (PCR sensitivity). It was shown that 1-10 ul microsphere suspensions (2 mg/ml) in PCR mixtures do not interfere in the PCR. It can be assumed that the magnetic nuclei were covered with polymer layer.

Keywords

magnetic microspheres, real time PCR

Authors

TRACHTOVÁ, Š.; RITTICH, B.; HORÁK, D.; ŠPANOVÁ, A.

Released

31. 10. 2012

Publisher

Institute of Analytical chemstry AS CR

Location

Brno, Czech Republic

ISBN

978-80-904959-1-3

Book

CECE 2012, 9th International Interdisciplinary Meeting on Bioanalysis

Edition

Edition number

Pages from

374

Pages to

378

Pages count

BibTex

@misc{BUT94933,
  author="Štěpánka {Trachtová} and Bohuslav {Rittich} and Daniel {Horák} and Alena {Španová}",
  title="The Use of Real Time PCR Method for Evaluation of Magnetic Microspheres",
  booktitle="CECE 2012, 9th International Interdisciplinary Meeting on Bioanalysis",
  year="2012",
  series="1",
  edition="1",
  pages="374--378",
  publisher="Institute of Analytical chemstry AS CR",
  address="Brno, Czech Republic",
  isbn="978-80-904959-1-3",
  note="abstract"
}