PHA-Based Particles Prepared by Nanoprecipitation as Drug Carriers

abstract

English

Biodegradable polymeric particles represent an interesting approach for in vivo delivery of drugs, vaccine antigens, or hormones [1]. When administered, they distribute the drug more uniformly than single unit administration, which reduces local irritation as well. In polymeric particles, active substances can be entrapped, dissolved, or adhere to the surface [2]. Such systems generally use three different described mechanisms to release the encapsulated substances, including monophasic release with diffusion; biphasic release with a high initial burst followed by saturation; triphasic profile starting an initial burst, then polymer degradation and ending with a slow diffusion release [3]. The efficiency of the performance of particles in the body also depends on their size, as smaller sizes increase their intracellular uptake and mobility to the target sites [2]. Various top-down and bottom-up methods are reported for the preparation of polymeric particles [4,5], and nanoprecipitation is one of them [6]. This method allows changing the size of the obtained polymer particles very easily by changing processing parameters, such as temperature, stirring rate, or polymer concentration. Polyhydroxyalkanoates (PHAs) have great potential in this area, especially due to their biocompatibility, biodegradability, and their wide range of mechanical properties [7]. Besides, their hydrophobicity is an advantage, as the intestinal uptake of hydrophobic polymers, when used as drug delivery carriers, was higher than the hydrophilic ones [8,9]. Herein, we have designed drug delivery carriers based on different types of PHAs. The effect of varying the parameters of nanoprecipitation as a preparation method on the resulting particle sizes has been described. This work shows the release kinetics of the encapsulated substances studied in different environments. The tests induced different pH values, which also vary naturally in the human body depending on the site. The effect of acidic and neutral environments on release was compared.

PHA, nanoprecipitation, nanoparticles

ČERNEKOVÁ, N.; ČECH, J.; KOVALČÍK, A.

13. 9. 2023

Brno, Czech Republic

140

140

163

https://esbp2023.com/src/ESBP2023_Book_of_Abstracts.pdf