3D Scaffolds Based on Bacterial Cellulose

abstract

English

Bacterial cellulose is an extracellular polysaccharide formed as a protective envelope around certain types of bacterial cells. The application of bacterial cellulose has been explored in many areas, such as the food industry, medicine, biotechnology, cosmetics, electronics, and textile industry. Several cultivation methods have been investigated to enhance bacterial cellulose production and decrease its cultivation costs [1]. This work involves optimizing bacterial cellulose by modifying the production medium and subsequently preparing 3D samples of bacterial cellulose to increase its mechanical stability. It has been found that an increase in bacterial cellulose production can be achieved by modifying the surface of the gas‑liquid interface, such as by aeration, the addition of acetate buffer, or oil. Active aeration increased bacterial cellulose production by 235%. Next, the effect of using fed‑batch cultivation compared to static batch cultivation was also verified. In this work, several cultivation strategies have been employed to produce stable mechanical 3D bacterial cellulose scaffolds in‑situ during the cultivation of Komagateibacter xylinus. The most effective methodologies enabling bacterial cellulose production in the form of cylinders with a height of up to 2 cm were: 1) the static cultivation of K. xylinus inside the stable foam [2] or 2) cultivation in Hestrin‑Scharmm medium with the addition of polyvinyl alcohol. The morphological investigations have shown that the bacterial cellulose’s porosity varies significantly depending on the applied cultivation methodology. It can be concluded that porosity is an important parameter that affects the absorption of water and the viscoelastic properties of bacterial cellulose scaffolds. The advantage of scaffolds with variable porosity based on bacterial cellulose is their non‑toxicity, biocompatibility and the possibility of gradual release of the bioactive substance or drug.

Bacterial cellulose; scaffolds

STŘÍŽ, R.; ČERNEKOVÁ, N.; SMILEK, J.; LIEBNER, F.; BOBER, P.; KOVALČÍK, A.

25. 11. 2021

Vysoké učení technické v Brně, Fakulta chemická

Brno

978-80-214-6002-7

Studentská odborná konference. CHEMIE JE ŽIVOT 2021 25.–26. 11. 2021. Sborník abstraktů

58

60

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