Publication detail

Healing and Angiogenic Properties of Collagen/Chitosan Scaffolds Enriched with Hyperstable FGF2-STAB(R) Protein: In Vitro, Ex Ovo and In Vivo Comprehensive Evaluation

VOJTOVÁ, L. PAVLIŇÁKOVÁ, V. MUCHOVÁ, J. KACVINSKÁ, K. BRTNÍKOVÁ, J. KNOZ, M. LIPOVÝ, B. FALDYNA, M. GÖPFERT, E. HOLOUBEK, J. PAVLOVSKÝ, Z. VÍCENOVÁ, M. BLAHNOVÁ, V. HEARNDEN, V. FILOVA, E.

Original Title

Healing and Angiogenic Properties of Collagen/Chitosan Scaffolds Enriched with Hyperstable FGF2-STAB(R) Protein: In Vitro, Ex Ovo and In Vivo Comprehensive Evaluation

Type

journal article in Web of Science

Language

English

Original Abstract

Wound healing is a process regulated by a complex interaction of multiple growth factors including fibroblast growth factor 2 (FGF2). Although FGF2 appears in several tissue engineered studies, its applications are limited due to its low stability both in vitro and in vivo. Here, this shortcoming is overcome by a unique nine-point mutant of the low molecular weight isoform FGF2 retaining full biological activity even after twenty days at 37 degrees C. Crosslinked freeze-dried 3D porous collagen/chitosan scaffolds enriched with this hyper stable recombinant human protein named FGF2-STAB(R) were tested for in vitro biocompatibility and cytotoxicity using murine 3T3-A31 fibroblasts, for angiogenic potential using an ex ovo chick chorioallantoic membrane assay and for wound healing in vivo with 3-month old white New Zealand rabbits. Metabolic activity assays indicated the positive effect of FGF2-STAB(R) already at very low concentrations (0.01 mu g/mL). The angiogenic properties examined ex ovo showed enhanced vascularization of the tested scaffolds. Histological evaluation and gene expression analysis by RT-qPCR proved newly formed granulation tissue at the place of a previous skin defect without significant inflammation infiltration in vivo. This work highlights the safety and biocompatibility of newly developed crosslinked collagen/chitosan scaffolds involving FGF2-STAB(R) protein. Moreover, these sponges could be used as scaffolds for growing cells for dermis replacement, where neovascularization is a crucial parameter for successful skin regeneration.

Keywords

collagen; chitosan; scaffold; FGF2; skin regeneration; tissue engineering

Authors

VOJTOVÁ, L.; PAVLIŇÁKOVÁ, V.; MUCHOVÁ, J.; KACVINSKÁ, K.; BRTNÍKOVÁ, J.; KNOZ, M.; LIPOVÝ, B.; FALDYNA, M.; GÖPFERT, E.; HOLOUBEK, J.; PAVLOVSKÝ, Z.; VÍCENOVÁ, M.; BLAHNOVÁ, V.; HEARNDEN, V.; FILOVA, E.

Released

1. 6. 2021

Publisher

MDPI

Location

BASEL

ISBN

2227-9059

Periodical

Biomedicines

Year of study

9

Number

6

State

Swiss Confederation

Pages from

1

Pages to

27

Pages count

27

URL

https://www.mdpi.com/2227-9059/9/6/590

Full text in the Digital Library

http://hdl.handle.net/11012/200493

BibTex

@article{BUT171955,
  author="Lucy {Vojtová} and Veronika {Pavliňáková} and Johana {Muchová} and Katarína {Verčimáková} and Jana {Brtníková} and Martin {Knoz} and Břetislav {Lipový} and Martin {Faldyna} and Eduard {Göpfert} and Jakub {Holoubek} and Zdeněk {Pavlovský} and Monika {Vícenová} and Veronika {Blahnová} and Vanessa {Hearnden} and Eva {Filova}",
  title="Healing and Angiogenic Properties of Collagen/Chitosan Scaffolds Enriched with Hyperstable FGF2-STAB(R) Protein: In Vitro, Ex Ovo and In Vivo Comprehensive Evaluation",
  journal="Biomedicines",
  year="2021",
  volume="9",
  number="6",
  pages="1--27",
  doi="10.3390/biomedicines9060590",
  issn="2227-9059",
  url="https://www.mdpi.com/2227-9059/9/6/590"
}