Publication detail

Evaluating the Influence of a G-Quadruplex Prone Sequence on the Transactivation Potential by Wild-Type and/or Mutant P53 Family Proteins through a Yeast-Based Functional Assay

MONTI, P. BRÁZDA, V. BOHÁLOVÁ, N. PORUBIAKOVÁ, O. MENICHINI, P. SPECIALE, A. BOCCIARDI, R. INGA, A. FRONZA, G.

Original Title

Evaluating the Influence of a G-Quadruplex Prone Sequence on the Transactivation Potential by Wild-Type and/or Mutant P53 Family Proteins through a Yeast-Based Functional Assay

Type

journal article in Web of Science

Language

English

Original Abstract

P53, P63, and P73 proteins belong to the P53 family of transcription factors, sharing a common gene organization that, from the P1 and P2 promoters, produces two groups of mRNAs encoding proteins with different N-terminal regions; moreover, alternative splicing events at Cterminus further contribute to the generation of multiple isoforms. P53 family proteins can influence a plethora of cellular pathways mainly through the direct binding to specific DNA sequences known as response elements (REs), and the transactivation of the corresponding target genes. However, the transcriptional activation by P53 family members can be regulated at multiple levels, including the DNA topology at responsive promoters. Here, by using a yeast-based functional assay, we evaluated the influence that a G-quadruplex (G4) prone sequence adjacent to the p53 RE derived from the apoptotic PUMA target gene can exert on the transactivation potential of full-length and Nterminal truncated P53 family α isoforms (wild-type and mutant). Our results show that the presence of a G4 prone sequence upstream or downstream of the P53 RE leads to significant changes in the relative activity of P53 family proteins, emphasizing the potential role of structural DNA features as modifiers of P53 family functions at target promoter sites.

Keywords

p53 family, yeast, G-quadruplex(G4) prone sequence, wild-type and mutant p53/p63 proteins, transactivation potential

Authors

MONTI, P.; BRÁZDA, V.; BOHÁLOVÁ, N.; PORUBIAKOVÁ, O.; MENICHINI, P.; SPECIALE, A.; BOCCIARDI, R.; INGA, A.; FRONZA, G.

Released

15. 2. 2021

Publisher

MDPI

Location

Basel, Switzerland

ISBN

2073-4425

Periodical

Genes

Year of study

12

Number

2

State

Swiss Confederation

Pages from

1

Pages to

13

Pages count

13

URL

https://www.mdpi.com/2073-4425/12/2/277

Full text in the Digital Library

http://hdl.handle.net/11012/196738

BibTex

@article{BUT169563,
  author="Paola {Monti} and Václav {Brázda} and Natália {Bohálová} and Otília {Porubiaková} and Paola {Menichini} and Andrea {Speciale} and Renata {Bocciardi} and Alberto {Inga} and Gilberto {Fronza}",
  title="Evaluating the Influence of a G-Quadruplex Prone Sequence on the Transactivation Potential by Wild-Type and/or Mutant P53 Family Proteins through a Yeast-Based Functional Assay",
  journal="Genes",
  year="2021",
  volume="12",
  number="2",
  pages="1--13",
  doi="10.3390/genes12020277",
  issn="2073-4425",
  url="https://www.mdpi.com/2073-4425/12/2/277"
}