Publication detail

Metallothionein dimerization evidenced by QD-based Förster resonance energy transfer and capillary electrophoresis

PAVELICOVÁ, K. POMPEIANO VANÍČKOVÁ, L. HADDAD, Y. NEJDL, L. ZÍTKA, J. KOČIOVÁ, S. MRAVEC, F. VACULOVIČ, T. MACKA, M. VACULOVIČOVÁ, M. ADAM, V.

Original Title

Metallothionein dimerization evidenced by QD-based Förster resonance energy transfer and capillary electrophoresis

Type

journal article in Web of Science

Language

English

Original Abstract

Herein, we report a new simple and easy-to-use approach for the characterization of protein oligomerization based on fluorescence resonance energy transfer (FRET) and capillary electrophoresis with LED-induced detection. The FRET pair consisted of quantum dots (QDs) used as an emission tunable donor (emission wavelength of 450 nm) and a cyanine dye (Cy3), providing optimal optical properties as an acceptor. Nonoxidative dimerization of mammalian metallothionein (MT) was investigated using the donor and acceptor covalently conjugated to MT. The main functions of MTs within an organism include the transport and storage of essential metal ions and detoxification of toxic ions. Upon storage under aerobic conditions, MTs form dimers (as well as higher oligomers), which may play an essential role as mediators in oxidoreduction signaling pathways. Due to metal bridging by Cd2+ ions between molecules of metallothionein, the QDs and Cy3 were close enough, enabling a FRET signal. The FRET efficiency was calculated to be in the range of 11–77%. The formation of MT dimers in the presence of Cd2+ ions was confirmed by MALDI-MS analyses. Finally, the process of oligomerization resulting in FRET was monitored by CE, and oligomerization of MT was confirmed.

Keywords

FRET;Quantum dots;Oligomerization;Capillary electrophoresis

Authors

PAVELICOVÁ, K.; POMPEIANO VANÍČKOVÁ, L.; HADDAD, Y.; NEJDL, L.; ZÍTKA, J.; KOČIOVÁ, S.; MRAVEC, F.; VACULOVIČ, T.; MACKA, M.; VACULOVIČOVÁ, M.; ADAM, V.

Released

15. 2. 2021

Publisher

Elsevier

Location

Nizozemsko

ISBN

0141-8130

Periodical

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Year of study

170

Number

1

State

Kingdom of the Netherlands

Pages from

53

Pages to

60

Pages count

8

URL

https://www.sciencedirect.com/science/article/pii/S0141813020352466

Full text in the Digital Library

http://hdl.handle.net/11012/208291

BibTex

@article{BUT167427,
  author="Kristýna {Pavelicová} and Lucie {Pompeiano Vaníčková} and Yazan Abdulmajeed Eyadh {Haddad} and Lukáš {Nejdl} and Jan {Zítka} and Silvia {Kočiová} and Filip {Mravec} and Tomáš {Vaculovič} and Mirek {Macka} and Markéta {Vaculovičová} and Vojtěch {Adam}",
  title="Metallothionein dimerization evidenced by QD-based Förster resonance energy transfer and capillary electrophoresis",
  journal="INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES",
  year="2021",
  volume="170",
  number="1",
  pages="53--60",
  doi="10.1016/j.ijbiomac.2020.12.105",
  issn="0141-8130",
  url="https://www.sciencedirect.com/science/article/pii/S0141813020352466"
}