Thermophilic Bacterium Schlegelella thermodepolymerans DSM 15344 as a Producer of Polyhydroxyalkanoates

abstract

English

Thermophilic microorganisms grow and thrive even at temperatures that are inhibitory or even lethal to most microorganisms. In the last few years, thermophilic microorganisms have also been considered as production cultures for other substances, e.g. polyhydroxyalkanoates. Polyhydroxyalkanoates (PHAs) are biodegradable and biocompatible polyesters that are produced and accumulated by bacteria as intracellular granules. These biopolymers have similar properties to some synthetic plastics but are not harmful to the environment. Unfortunately, their production cost is still high. Therefore, approaches are being sought to produce PHAs cheaper. One of the representatives of thermophilic microorganisms is the gram-negative bacterium Schlegelella thermodepolymerans DSM 15344. We began to investigate its biotechnological potential. Production conditions were tested at various temperatures, carbon substrates and precursors suitable for the synthesis of polymers with modified properties. S. thermodepolymerans is able to utilize xylose very well. Since xylose is present in numerous lignocellulosic materials, we cultured the bacterium also on model hydrolysates and we also evaluated its sensitivity to microbial inhibitors present in lignocellulose hydrolysates. According to our results, S. thermodepolymerans appears to be a very promising producer of PHAs.

Schlegelella thermodepolymerans, thermophiles, polyhydroxyalkanoates, biopolymers

KOUŘILOVÁ, X.; MUSILOVÁ, J.; SEDLÁŘ, K.; BEDNÁROVÁ, K.; PERNICOVÁ, I.; OBRUČA, S.

27. 11. 2020

Vysoké učení technické v Brně, Fakulta chemická

Brno

978-80-214-5920-0

Studentská odborná konference CHEMIE JE ŽIVOT 2020

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