Publication detail

Application of osmotic challenge for enrichment of microbial consortia in polyhydroxyalkanoates producing thermophilic and thermotolerant bacteria and their subsequent isolation

PERNICOVÁ, I. NOVÁČKOVÁ, I. SEDLÁČEK, P. KOUŘILOVÁ, X. KOLLER, M. OBRUČA, S.

Original Title

Application of osmotic challenge for enrichment of microbial consortia in polyhydroxyalkanoates producing thermophilic and thermotolerant bacteria and their subsequent isolation

Type

journal article in Web of Science

Language

English

Original Abstract

Polyhydroxyalkanoates are microbial polyesters which are considered being biological alternatives to petrochemical polymers. Extremophiles, such as thermophilic PHA producers, hold promise to improve competitiveness of PHA production process. Therefore, this work aimed at isolation of new strains, which could produce PHA under elevated temperature. Since traditional Nile red staining of colonies provided false positive results in thermophiles, we developed a novel strategy of enriching microbial consortia by PHA producers. This so called “osmoselective strategy” is based on application of osmotic challenge by sudden exposition of the mixed microbial culture to hypertonic and subsequently to hypotonic conditions; moreover, this strategy relies on the fact that PHA protect bacteria from negative effects of rapid fluctuations in osmotic pressure. In combination with fast and reliable ATR-FTIR inspection of selected colonies for presence of PHA, we were able to isolate several promising thermophilic or thermotolerant PHA producing strains belonging to the genera Bacillus, Aneurinibacillus and Chelatococcus, which indeed deserves further investigation to evaluate their potential for industrial production of PHA.

Keywords

Polyhydroxyalkanoates; Enrichment; Isolation of bacteria; Osmotic challenge; Thermophiles

Authors

PERNICOVÁ, I.; NOVÁČKOVÁ, I.; SEDLÁČEK, P.; KOUŘILOVÁ, X.; KOLLER, M.; OBRUČA, S.

Released

1. 2. 2020

Publisher

Elsevier

ISBN

0141-8130

Periodical

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Year of study

144

Number

2

State

Kingdom of the Netherlands

Pages from

698

Pages to

704

Pages count

7

URL

https://www.sciencedirect.com/science/article/pii/S0141813019388208

BibTex

@article{BUT161204,
  author="PERNICOVÁ, I. and NOVÁČKOVÁ, I. and SEDLÁČEK, P. and KOUŘILOVÁ, X. and KOLLER, M. and OBRUČA, S.",
  title="Application of osmotic challenge for enrichment of microbial consortia in polyhydroxyalkanoates producing thermophilic and thermotolerant bacteria and their subsequent isolation",
  journal="INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES",
  year="2020",
  volume="144",
  number="2",
  pages="698--704",
  doi="10.1016/j.ijbiomac.2019.12.128",
  issn="0141-8130",
  url="https://www.sciencedirect.com/science/article/pii/S0141813019388208"
}