Publication detail

SIMULTANEOUS DETECTION OF PEACH AND APRICOT DNA BY MULTIPLEX REAL-TIME PCR-HRM WITH INTERCALATING DYE

FIALOVÁ, L. ROMANOVSKÁ, D. MÁROVÁ, I.

Original Title

Type

conference proceedings

Language

English

Original Abstract

Multiplex real-time PCR-HRM with specific primers and intercalating dye was suggested as a new method for authentication of products containing buffalo meat by Sakaridis et al [1]. It has since been used for authentication of other meat products and medicinal plant products, but not for authentication of fruit-based foodstuffs. In this work we tested this method with DNA isolated from peach and apricot fruits and with two primer pairs, whose specifity was verified by real-time PCR‐HRM [2, 3]. The multiplex real‐time PCR‐HRM was carried out with equal amounts of DNA from both fruit species, and its sensitivity was tested with decreasing amounts of each DNA (1 ng, 100 pg, 10 pg, 1 pg). We detected peach DNA in assays with 1 ng to 10 pg of DNA, and apricot DNA was detected in assays with 1 ng to 100 pg of DNA. We were able to detect two disctinct amplicones in all assays in which both peach and apricot DNA was detected.

Keywords

multiplex PCR; real-time PCR-HRM; intercalating dye; peach; apricot

Authors

FIALOVÁ, L.; ROMANOVSKÁ, D.; MÁROVÁ, I.

Released

24. 9. 2019

BibTex

@proceedings{BUT160121,
  editor="Lenka {Fialová} and Denisa {Langová} and Ivana {Márová}",
  title="SIMULTANEOUS DETECTION OF PEACH AND APRICOT DNA BY MULTIPLEX REAL-TIME PCR-HRM WITH INTERCALATING DYE",
  year="2019"
}