Publication detail
Adaptation of Cupriavidus necator H16 to levulinic acid for enhancement of 3-hydroxyvalerate content in copolymer P(3HB-co-3HV)
NOVÁČKOVÁ, I. KUČERA, D. POŘÍZKA, J. PERNICOVÁ, I. SEDLÁČEK, P. OBRUČA, S.
Original Title
Adaptation of Cupriavidus necator H16 to levulinic acid for enhancement of 3-hydroxyvalerate content in copolymer P(3HB-co-3HV)
Type
abstract
Language
English
Original Abstract
Within this work we used methods of evolutionary engineering to gain strains adapted to levulinic acid able to growth at the presence of levulinic acid and to convert it to 3HV and incorporate into copolymer P(3HB-co-3HV) more than wild-type strain. On the basis of specific enzyme activities of selected enzymes through metabolism of evolved strains we tried to suggest possible metabolic adaptation strategies and we also used methods of statistical analysis such as PCA and AHC for searching of common and different strategies between evolved strains.
Keywords
evolutionary engineering, levulinic acid, P(3HB-co-3HV)
Authors
NOVÁČKOVÁ, I.; KUČERA, D.; POŘÍZKA, J.; PERNICOVÁ, I.; SEDLÁČEK, P.; OBRUČA, S.
Released
13. 11. 2018
Location
Brno
ISBN
978-80-210-9069-9
Book
XIX. setkání biochemiků a molekulárních biologů Wimmerová, Michaela, Holková, Jitka, eds.
Pages from
76
Pages to
76
Pages count
1
BibTex
@misc{BUT151417,
author="Ivana {Nováčková} and Dan {Kučera} and Jaromír {Pořízka} and Iva {Buchtíková} and Petr {Sedláček} and Stanislav {Obruča}",
title="Adaptation of Cupriavidus necator H16 to levulinic acid for enhancement of 3-hydroxyvalerate content in copolymer P(3HB-co-3HV)",
booktitle="XIX. setkání biochemiků a molekulárních biologů Wimmerová, Michaela, Holková, Jitka, eds.",
year="2018",
pages="76--76",
address="Brno",
isbn="978-80-210-9069-9",
note="abstract"
}