Publication detail

Dual-color quantum dots-based simultaneous detection of HPV-HIV co-infection

JIMENEZ JIMENEZ, A. MOULICK, A. RICHTERA, L. KREJČOVÁ, L. KALINA, L. DATTA, R. SVOBODOVÁ, M. HYNEK, D. MASAŘÍK, M. HEGER, Z. ADAM, V.

Original Title

Type

journal article in Web of Science

Language

English

Original Abstract

Human papillomavirus (HPV) and human immunodeficiency virus (HIV) are closely related infections; both diseases are mutually reinforcing due to the co-infection effects, which increase their clinical and epidemiological implications, raising urgent public health concerns. In the present study, a nano-system was developed for simultaneous detection of HPV and HIV targeting E6-HPV16 and Gag-HIV genes respectively. The target sequences from the samples of the patients suffering from head and neck cancer were isolated by magnetic glass particles (MGPs) and subsequently were hybridized with a mixture of the complementary probes attached to cadmium telluride quantum dots (CdTe QDs). Finally, the specific and successful binding was determined by an easy fluorescence analyses and was corroborated by PCR and electrochemistry. Red and green CdTe QDs were used for the dual-color detection of HPV and HIV respectively. Our nano-system can be applied as a proficient tool for the identification of HPV, HIV or both diseases simultaneously, getting a successful and high sensitivity system of detection.

Keywords

Electrochemistry; Human immunodeficiency virus; Human papillomavirus; Nanotechnology; Magnetic isolation; Quantum dots

Authors

JIMENEZ JIMENEZ, A.; MOULICK, A.; RICHTERA, L.; KREJČOVÁ, L.; KALINA, L.; DATTA, R.; SVOBODOVÁ, M.; HYNEK, D.; MASAŘÍK, M.; HEGER, Z.; ADAM, V.

Released

1. 4. 2018

ISBN

0925-4005

Periodical

Sensors and Actuators B: Chemical

Year of study

258

Number

State

Swiss Confederation

Pages from

295

Pages to

303

Pages count

BibTex

@article{BUT146945,
  author="Ana Maria {Jimenez Jimenez} and Amitava {Moulick} and Lukáš {Richtera} and Ludmila {Krejčová} and Lukáš {Kalina} and Rahul {Datta} and Markéta {Svobodová} and David {Hynek} and Michal {Masařík} and Zbyněk {Heger} and Vojtěch {Adam}",
  title="Dual-color quantum dots-based simultaneous detection of HPV-HIV co-infection",
  journal="Sensors and Actuators B: Chemical",
  year="2018",
  volume="258",
  number="1",
  pages="295--303",
  doi="10.1016/j.snb.2017.11.074",
  issn="0925-4005"
}