Publication detail

Simultaneous production of lipids and pigments by red yeasts grown on animal fat using the concept of biorefinery

MÁROVÁ, I. SZOTKOWSKI, M. RAPTA, M. BYRTUSOVÁ, D. VANĚK, M. HÁRONIKOVÁ, A. MIKHEICHYK, N.

Original Title

Simultaneous production of lipids and pigments by red yeasts grown on animal fat using the concept of biorefinery

Type

article in a collection out of WoS and Scopus

Language

English

Original Abstract

Characterization of metabolic activity of yeasts in biotechnological applications is an important issue both for routine analysis and trouble-shooting incidences. In this work 12 different strains of red yeasts (Rhodotorula, Sporobolomyces, Cystofilobasidium) were used for screening of growth and metabolic activity. Simultaneous production of lipids and other lipid metabolites was followed. As a carbon substrate waste animal fat and its hydrolysis products were used according to the biorefinery concept. Yeasts were cultivated in medium with particular C/N ratio. Whey was used as alternative nitrogen source. High-throughput micro-cultivation, FTIR spectroscopy and single cell analysis of red yeasts was used for monitoring of growth characteristics and total metabolic activity of yeasts. Simultaneously, chromatographic techniques were used for quantification of production of lipids and fatty acids (GC/FID), sterols, carotenoids and CoQ (LC/MS). Glycerol, lipase and total lipids were measured by enzyme methods. Cells were monitored by advanced fluorescence techniques on single-cell level.

Keywords

biorefinery, red yeasts, animal fat

Authors

MÁROVÁ, I.; SZOTKOWSKI, M.; RAPTA, M.; BYRTUSOVÁ, D.; VANĚK, M.; HÁRONIKOVÁ, A.; MIKHEICHYK, N.

Released

18. 10. 2017

Publisher

Formatex Research Centre

Location

Madrid, Spain

Pages from

199

Pages to

202

Pages count

345

URL

http://biomicroworld2017.org/

BibTex

@inproceedings{BUT145815,
  author="Ivana {Márová} and Martin {Szotkowski} and Marek {Rapta} and Dana {Byrtusová} and Martin {Vaněk} and Andrea {Němcová} and Nadzeya {Mikheichyk}",
  title="Simultaneous production of lipids and pigments by red yeasts grown on animal fat using the concept of biorefinery",
  year="2017",
  pages="199--202",
  publisher="Formatex Research Centre",
  address="Madrid, Spain",
  url="http://biomicroworld2017.org/"
}