Publication detail

The study of biocompatibility of magnetic particles using amplification methods

TRACHTOVÁ, Š. ŠPANOVÁ, A. HORÁK, D. HLÍDKOVÁ, H. KOSTIV, U. RITTICH, B.

Original Title

Type

abstract

Language

English

Original Abstract

Magnetic particles for biotechnology applications usually consist of a number of components: magnetic cores are covered with a variety of functionalized low-or high- molecular compounds (polymer layer). Iron oxides and some substances used to stabilize magnetic cores can reduce biocompatibility of the proposed particles; therefore perfectly encapsulated cores are very important for their application. The real-time polymerase chain reaction (qPCR) is very sensitive for the presence a number of chemicals. The increasing inhibitors concentration decreases the amplification efficiency and thus the reaction curve slope decreases. The experiments for the estimation magnetic nano- and microparticles inhibitory effect or biocompatibility respectively on the course of real-time PCR were proposed. The set of magnetic non-porous microspheres covered by different content of carboxyl groups, PEGylated monodisperse magnetic poly(2-hydroxyethyl methacrylate) and poly(glycidyl methacrylate) microspheres, newly synthetized types of particles - lanthanide-doped up converting nanoparticles -NaYF4:Yb3+/Er3+ particles and compounds used for magnetic core encapsulation were studied. Linear regression analyses were used for the evaluation of the influence of the particles or compounds on the qPCR course. It was confirmed that the qPCR can be used for the study of compatibility of magnetic particles with DNA amplification. Real-time PCR methods based on shift of Cq values after addition of different amounts of the particles to DNA was found as a suitable method for evaluation of their inhibition effect.

Keywords

biocompatibility. magnetic nanoparticles, real-time PCR

Authors

TRACHTOVÁ, Š.; ŠPANOVÁ, A.; HORÁK, D.; HLÍDKOVÁ, H.; KOSTIV, U.; RITTICH, B.

Released

2. 9. 2015

Location

Brno, Czech Republic

Pages from

168

Pages to

168

Pages count

URL

http://www.ceitec.eu/a5-abstracts-book-3d/f33102

BibTex

@misc{BUT133349,
  author="Štěpánka {Trachtová} and Alena {Španová} and Daniel {Horák} and Helena {Hlídková} and Uliana {Kostiv} and Bohuslav {Rittich}",
  title="The study of biocompatibility of magnetic particles using amplification methods",
  year="2015",
  pages="168--168",
  address="Brno, Czech Republic",
  url="http://www.ceitec.eu/a5-abstracts-book-3d/f33102",
  note="abstract"
}