Publication detail

A novel in situ Silver/Hyaluronan Bio-nanocomposite Fabrics for Wound and Chronic Ulcer Dressing: In Vitro and In Vivo Evaluations

ABDEL-LATTIF, A. JANČÁŘ, J. ABDELRAHMAN, R. VOJTEK, L. HYRŠL, P. DUŠKOVÁ, M. NEJEZCHLEBOVÁ, H.

Original Title

A novel in situ Silver/Hyaluronan Bio-nanocomposite Fabrics for Wound and Chronic Ulcer Dressing: In Vitro and In Vivo Evaluations

Type

journal article in Web of Science

Language

English

Original Abstract

In-situ formed hyaluronan/silver (HA/Ag) nanoparticles (NPs) were used to prepare composite fibers/fabrics for the first time. Different concentrations of silver nitrate (1, 2 mg/100 ml) were added at ambient temperature to sodium hyaluronate solution (40 mg/ml), then the pH was increased to 8 by adding sodium hydroxide. The in-situ formed HA/Ag-NPs were used to prepare fibers/nonwoven fabrics by wet-dry-spinning technique (WDST). UV/vis spectroscopy, SEM, TEM, DLS, XPS, XRD and TGA were employed to characterize the structure and composition of the nanocomposite, surface morphology of fiber/fabrics, particle size of Ag-NPs, chemical interactions of Ag0 and HA functional groups, crystallinity and thermal stability of the wound dressing, respectively. The resultant HA/Ag-NPs1 and HA/Ag-NPs2 composite showed uniformly dispersed throughout HA fiber/fabrics (SEM), an excellent distribution of Ag-NPs with 25 ± 2, nm size (TEM, DLS) and acceptable mechanical properties. The XRD analysis showed that the in-situ preparation of Ag-NPs increased the crystallinity of the resultant fabrics as well as the thermal stability. The antibacterial performance of medical HA/Ag-NPs fabrics were evaluated against gram negative bacteria E. coli K12, exhibiting significant bactericidal activity. The fibers did not show any cytotoxicity against human keratinocyte cell line (HaCaT). In-vivo animal tests indicated that the prepared wound dressing has strong healing efficacy (non-diabetics/diabetics rat model) compared to the plain HA fabrics and greatly accelerated the healing process. Based on our results, the new HA/Ag-NPs-2 mg nonwoven wound dressing fabrics can be used in treating wounds and chronic ulcers as well as cell carrier in different biological research and tissue engineering.

Keywords

In-situ silver, hyaluronan, cytotoxicity, wound dressing

Authors

ABDEL-LATTIF, A.; JANČÁŘ, J.; ABDELRAHMAN, R.; VOJTEK, L.; HYRŠL, P.; DUŠKOVÁ, M.; NEJEZCHLEBOVÁ, H.

Released

30. 3. 2017

ISBN

0378-5173

Periodical

INTERNATIONAL JOURNAL OF PHARMACEUTICS

Year of study

520

Number

241-253

State

Kingdom of the Netherlands

Pages from

120

Pages to

133

Pages count

51

URL

https://www.sciencedirect.com/science/article/abs/pii/S0378517317300844

BibTex

@article{BUT132823,
  author="Abdelmohsen Moustafa {Abdellatif} and Josef {Jančář} and Rasha {Radwan} and Libor {Vojtek} and Pavel {Hyršl} and marie {Dušková} and helena {Nejezchlebová}",
  title="A novel in situ Silver/Hyaluronan Bio-nanocomposite Fabrics for Wound and Chronic Ulcer Dressing: In Vitro and In Vivo Evaluations",
  journal="INTERNATIONAL JOURNAL OF PHARMACEUTICS",
  year="2017",
  volume="520",
  number="241-253",
  pages="120--133",
  doi="10.1016/j.ijpharm.2017.02.003",
  issn="0378-5173",
  url="https://www.sciencedirect.com/science/article/abs/pii/S0378517317300844"
}