Publication detail

Comparison of flow cytometry and Raman spectroscopy as methods for rapid detremination of intracellular content of polyhydroxyalkanoates in bacterial cells

KUČERA, D. SAMEK, O. MÜLLEROVÁ, L. BENEŠOVÁ, P. OBRUČA, S.

Original Title

Comparison of flow cytometry and Raman spectroscopy as methods for rapid detremination of intracellular content of polyhydroxyalkanoates in bacterial cells

Type

conference paper

Language

English

Original Abstract

Polyhydroxyalkanoates (PHAs) are bacterial polyesters which are considered as biodegradable and environmentally-friendly alternative to petrochemical plastics. Biotechnological production of PHAs is complicated by the lack of fast and reliable analytical tool enabling rapid and sensitive determination of PHAs in bacterial cells during the biotechnological process. The most commonly employed method for quantitative analysis of PHAs is gas chromatography, nevertheless, labor intensity and time demands of this methods prevents its application for routine analysis of PHAs during biotechnological process. The aim of this work was to compare two analytical methods which could be used for determination of intracellular content in time-frame of 20 minutes - Flow cytometry and Raman spectroscopy.

Keywords

PHAs, Flow cytometry, Raman spectroscopy

Authors

KUČERA, D.; SAMEK, O.; MÜLLEROVÁ, L.; BENEŠOVÁ, P.; OBRUČA, S.

Released

25. 4. 2016

ISBN

978-80-86238-91-3

Book

4th International Conference on Chemical Technology ICCT 2016 Sborník příspěvků

Pages from

488

Pages to

492

Pages count

5

URL

https://dev8-admin.morbo.puxdesign.cz/Amca-ICCT/media/content/2016/Proceedings_ICCT2016.pdf

BibTex

@inproceedings{BUT130036,
  author="Dan {Kučera} and Ota {Samek} and Lucie {Müllerová} and Pavla {Benešová} and Stanislav {Obruča}",
  title="Comparison of flow cytometry and Raman spectroscopy as methods for rapid detremination of intracellular content of polyhydroxyalkanoates in bacterial cells",
  booktitle="4th International Conference on Chemical Technology ICCT 2016 Sborník příspěvků",
  year="2016",
  pages="488--492",
  isbn="978-80-86238-91-3",
  url="https://dev8-admin.morbo.puxdesign.cz/Amca-ICCT/media/content/2016/Proceedings_ICCT2016.pdf"
}