Publication detail

Turbidimetry and Fluorescence Study of Effect of Hyaluronan Addition to Cationic Micellar Solution

PILGROVÁ, T. PEKAŘ, M.

Original Title

Turbidimetry and Fluorescence Study of Effect of Hyaluronan Addition to Cationic Micellar Solution

Type

abstract

Language

English

Original Abstract

Hyaluronan as negatively charged polyelectrolyte can interact with positively charged surfactant micelles via electrostatic interactions to form core-shell like aggregates. These self-assembly systems can solubilize hydrophobic active substances and therefore, they are potential carriers in drug delivery applications. This study deals with electrostatic association of cationic micelles of CTAB or Septonex with hyaluronan. The cationic micelle/hyaluronan complexes were studied using turbidimetry and fluorescence spectroscopy method. Turbidimetric titration was chosen as an indicator of the loss of transmitted light intensity due to the scattering effect of particles associated from hyaluronan and surfactant in it. Fluorescent probe pyrene was selected for spectroscopy experiments because of its unique sensitivity to polarity of the medium, so this fluorescence method was used as an indicator of presence of hydrophobic domains in the system. Effect of components concentration and molecular weight of hyaluronan were evaluated. Results of turbidimetry revealed that aggregates formation (turbidity increasing) depends especially on a charge ratio of surfactant molecules and hyaluronan carboxyl groups. It was found a difference in association of low molecular weight and high molecular weight of hyaluronan. Fluorescence results confirmed presence and stability of micellar aggregates in studied systems.

Keywords

hyaluronan, surfactant, turbidimetry, fluorescence, pyrene

Authors

PILGROVÁ, T.; PEKAŘ, M.

Released

11. 9. 2016

Publisher

Leibniz-Institut für Polymerforschung Dresden e. V.

Location

Dresden, Germany

ISBN

978-3-9816007-4-2

Book

Book of Abstracts, 20th European Symposium on Polymer Spectroscopy (ESOPS)

Pages from

64

Pages to

64

Pages count

1

URL

http://www.ipfdd.de/fileadmin/user_upload/oea/00_Tagungsband_ESOPS20_2016-09-15.pdf

BibTex

@misc{BUT128282,
  author="Tereza {Pilgrová} and Miloslav {Pekař}",
  title="Turbidimetry and Fluorescence Study of Effect of Hyaluronan Addition to Cationic Micellar Solution",
  booktitle="Book of Abstracts,  20th European Symposium on Polymer Spectroscopy (ESOPS)",
  year="2016",
  pages="1",
  publisher="Leibniz-Institut für Polymerforschung Dresden e. V.",
  address="Dresden, Germany",
  isbn="978-3-9816007-4-2",
  url="http://www.ipfdd.de/fileadmin/user_upload/oea/00_Tagungsband_ESOPS20_2016-09-15.pdf",
  note="abstract"
}