Publication detail
DNA based identification of microorganisms in a probiotic products
ČAKAJDOVÁ, M. TRACHTOVÁ, Š. ŠPANOVÁ, A. HORÁK, D. RITTICH, B.
Original Title
DNA based identification of microorganisms in a probiotic products
Type
abstract
Language
English
Original Abstract
Brewers yeasts (Saccharomyces cerevisiae) are a secondary product from production of beer. They are used as a nutritional supplement for long time, too. Brewers yeasts can be supplemented with probiotic bacteria and roughage and with vitamins, minerals and enzymes, too. Brewers yeast tablets with content of genus Lactobacillus and Biffidobacterium were used for analysis. Two types of samples were before expiration, and other were after expiration (1 and 8 years). DNA was isolated from crude cell lysates of brewers yeast tablets using magnetics P(HEMA-co-GMA) microspheres covered by carboxyl groups. Non-diluted DNA was used for analysis of Lactobacillus and Biffidobacterium. The conventional polymerase chain reaction (PCR) and quantitative real-time PCR (qPCR) were used for amplification of DNAs.
Keywords
probiotic products, polymerase chain reaction, real time polymerase chain reaction, DNA isolation
Authors
ČAKAJDOVÁ, M.; TRACHTOVÁ, Š.; ŠPANOVÁ, A.; HORÁK, D.; RITTICH, B.
Released
28. 5. 2013
Publisher
Institute of Immunology and Experimental Therapy Polish academy of Science
Location
Kudowa Zdrój, Polsko
ISBN
978-83-928488-3-7
Book
Microbiology and Immunology of Mucosa Probiotics Conference 2013
Edition
1
Edition number
1
Pages from
41
Pages to
41
Pages count
1
BibTex
@misc{BUT99950,
author="Martina {Čakajdová} and Štěpánka {Trachtová} and Alena {Španová} and Daniel {Horák} and Bohuslav {Rittich}",
title="DNA based identification of microorganisms in a probiotic products",
booktitle="Microbiology and Immunology of Mucosa Probiotics Conference 2013",
year="2013",
series="1",
edition="1",
pages="41--41",
publisher="Institute of Immunology and Experimental Therapy Polish academy of Science",
address="Kudowa Zdrój, Polsko",
isbn="978-83-928488-3-7",
note="abstract"
}