Publication detail

Hydrophilic magnetic non-porous microspheres for molecular diagnostics applications

CHROBOKOVÁ, M. ŠPANOVÁ, A. TRACHTOVÁ, Š. HORÁK, D. RITTICH, B.

Original Title

Hydrophilic magnetic non-porous microspheres for molecular diagnostics applications

Type

abstract

Language

English

Original Abstract

The following applications of magnetic particles in analytical chemistry and molecular diagnostics are dominant: separation or purification of chemical species (e.g. nucleic acids or proteins); their use as carriers of enzymes for catalytical purposes in batch systems or as microreactors, and their application in sensors or biosensors. Magnetic nonporous hydrophilic microspheres poly(2-hydroxyethyl methacrylate-co-ethylene dimethacrylate)-(P(HEMA-co-EDMA)), poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate)–(P(HEMA-co-GMA)), and poly(glycidyl methacrylate)– (PGMA) were used as solid phase of enzymes for catalytical purposes in DNA analysis (such as RNase A, DNase I, proteinase K) and for specific and non-specific isolation of DNAs.

Keywords

magnetic particles, microorganisms, polymerase chain reaction, identification

Authors

CHROBOKOVÁ, M.; ŠPANOVÁ, A.; TRACHTOVÁ, Š.; HORÁK, D.; RITTICH, B.

Released

28. 5. 2013

Publisher

Institute of Immunolology and Experimental Therapy PASci

Location

Polsko

ISBN

978-83-928488-3-7

Book

Microbiology and Immunology of Mucosa Probiotics Conference 2013

Edition number

1

Pages from

43

Pages to

43

Pages count

1

BibTex

@misc{BUT99831,
  author="Maria {Chroboková} and Alena {Španová} and Štěpánka {Trachtová} and Daniel {Horák} and Bohuslav {Rittich}",
  title="Hydrophilic magnetic non-porous microspheres for molecular diagnostics applications",
  booktitle="Microbiology and Immunology of Mucosa Probiotics Conference 2013",
  year="2013",
  edition="1",
  pages="43--43",
  publisher="Institute of Immunolology and Experimental Therapy PASci",
  address="Polsko",
  isbn="978-83-928488-3-7",
  note="abstract"
}