Chiral Analysis of the Nonsteroidal Anti-inflammatory Drug Residuals in the Wastewater

abstract

English

Chiral pharmaceuticals are known to exhibit stereoselective behaviour when situated in a biological system (e.g. living organism) resulting in enantiomers of these compounds showing different biological properties such as toxicity. For better understanding of the fate of chiral pharmaceuticals in the environment it is of great importance to develop chiral analytical methods capable of trace analysis in the environmental matrices. Enantioselective HPLC-DAD method was developed for the analysis of two non-steroidal anti-inflammatory drugs: ibuprofen and ketoprofen. Enantiomers of these compounds were separated using Chiralpak AD as a chiral column in the normal phase mode. Extracted samples of raw and treated wastewater were at first analysed by achiral HPLC-ESI-MS method, which was used for quantification of the sum of ibuprofen and ketoprofen enantiomers. After this, the samples were analyzed by chiral HPLC using diode array detector. It was found out that the diode array detector is not sensitive and selective enough for the chiral analysis of wastewater samples and detection by mass spectrometry is needed. Since the developed chiral method is using normal phase liquid chromatography it is not compatible with electrospray ionization and atmospheric pressure chemical ionization should be used.

chirality, chiral pharmaceuticals, wastewater, HPLC, ibuprofen, ketoprofen, chiralpak AD

SVOBODOVÁ, D.; ČÁSLAVSKÝ, J.

22. 6. 2012

University of Nova Gorica

Slovenia

978-961-6311-70-0

Book of Abstracts: Thematic workshop - Instrumental analytical methods for environmenal monitoring

s26

s26

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