Publication detail
The use of enzymes contained in the washing powders to the preparation of the crude lyzates of Lactobacillus cells for magnetic DNA isolation
ČUTA, R. FRIČOVÁ, M. ŠPANOVÁ, A. RITTICH, B.
Original Title
The use of enzymes contained in the washing powders to the preparation of the crude lyzates of Lactobacillus cells for magnetic DNA isolation
Type
abstract
Language
English
Original Abstract
The preparation of crude lysates of bacterial cells is relatively expensive procedure because of the enzymes are used during the procedure. This problem can be solved by using of the washing powders for the cell lysis. The aim of our work was the isolation of DNA from the Lactobacillus at quality for the PCRs and to test whether the DNA isolated by the magnetic microsperes (P(HEMA-co-GMA) is appropriate to run the PCR with. We tried to find out if the composition of the washing powders is suitable for disruption of the cell wall instead of lysosyme.
Keywords
crude lyzates, Lactobacillus, isolation, DNA, PCR
Authors
ČUTA, R.; FRIČOVÁ, M.; ŠPANOVÁ, A.; RITTICH, B.
Released
17. 8. 2011
Publisher
Česká společnost chemická
Location
Praha
ISBN
0009-2770
Periodical
Chemické listy
Year of study
105
Number
18
State
Czech Republic
Pages from
s1009
Pages to
s1009
Pages count
1