Publication detail

Streptavidin-modified magnetic poly(2-hydroxyethyl methacrylate-co-g-lycidyl methacrylate) microspheres for selective isolation of bacterial DNA.

HORÁK, D., ŠPANOVÁ A., TVRDÍKOVÁ J.,RITTICH, B.

Original Title

Type

journal article in Web of Science

Language

English

Original Abstract

The dispersion copolymerization of 2-hydroxyethyl methacrylate (HEMA) with glycidyl methacrylate (GMA) in a toluene/2-methylpropan-1-ol mixture in the presence of Fe3O4 nanoparticles coated with oleic acid, produced monodisperse magnetic poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) (P(HEMA-GMA)) microspheres. Oxirane groups of the microspheres were ammonolyzed and then functionalized with streptavidin using cyanuric chloride. The final product contained 0.67 mg of streptavidin per gram of wet magnetic P(HEMA-GMA) microspheres. The microspheres were characterized by elemental analysis, scanning electron microscopy, IR, UV VIS and atomic absorption spectroscopy The streptavidin-modified magnetic P(HEMA-GMA) microspheres were used for immobilization of biotinylated DNA and subsequent selective isolation of target DNA from complex samples using DNA/DNA hybridisation.

Keywords

Glycidyl methacrylate; 2-hydroxyethyl methacrylate; Streptavidin; Biotin; Magnetic Microspheres; DNA

Authors

HORÁK, D., ŠPANOVÁ A., TVRDÍKOVÁ J.,RITTICH, B.

RIV year

2011

Released

30. 5. 2011

Publisher

PERGAMON-ELSEVIER SCIENCE

Location

Oxford

ISBN

0014-3057

Periodical

European Polymer Journal

Year of study

Number

State

United Kingdom of Great Britain and Northern Ireland

Pages from

1090

Pages to

1096

Pages count

BibTex

@article{BUT73688,
  author="Bohuslav {Rittich} and Alena {Španová} and Jana {Tvrdíková}",
  title="Streptavidin-modified magnetic poly(2-hydroxyethyl methacrylate-co-g-lycidyl methacrylate) microspheres for selective isolation of bacterial DNA.",
  journal="European Polymer Journal",
  year="2011",
  volume="47",
  number="5",
  pages="1090--1096",
  issn="0014-3057"
}