Publication detail

Characterisation of potential probiotic Lactobacillus strains of human origin

ŠPANOVÁ, A.TURKOVÁ, K. DRÁB, V. ŠPANO, M. BURDYCHOVÁ, R. RITTICH, B.

Original Title

Type

abstract

Language

English

Original Abstract

Molecular biological methods (genus-specific PCR, species-specific PCRs, RAPD with primer M13, and rep-PCR with primer (GTG)5) were applied for the identification and discrimination of 31 strains of the Lactobacillus genus isolated from faecal samples of 30 breastfed infants. All strains were tested for bile salts and low pH tolerance, growth in milk, production of lactic acids in milk supplemented with yeast extract, and used in biochemical tests including production of beta-glucuronidase. Consequently, the presence of genes coding for decarboxylases was investigated using PCR. Finally, four strains of probiotic species were selected for use in the dairy industry and for other tests concerning their probiotic character.

Keywords

Lactobacillus, identification, PCR, RAPD, rep-PCR, probiotics, in vitro tests

Authors

ŠPANOVÁ, A.;TURKOVÁ, K.; DRÁB, V.; ŠPANO, M.; BURDYCHOVÁ, R.; RITTICH, B.

Released

14. 9. 2010

Publisher

University of Bologna

Location

Rimini, Itálie

Pages count

BibTex

@misc{BUT73407,
  author="ŠPANOVÁ, A. and TURKOVÁ, K. and DRÁB, V. and ŠPANO, M. and BURDYCHOVÁ, R. and RITTICH, B.",
  title="Characterisation of potential probiotic Lactobacillus strains of human origin",
  booktitle="14th International Biotechnology Symposium Abstract Book",
  year="2010",
  pages="1",
  publisher="University of Bologna",
  address="Rimini, Itálie",
  note="abstract"
}