Publication detail

Silica coated La0.75Sr0.25MnO3 nanoparticles for magnetically driven DNA isolation

TRACHTOVÁ, Š. KAMAN, O. ŠPANOVÁ, A. VEVERKA, P. POLLERT, E. RITTICH, B.

Original Title

Silica coated La0.75Sr0.25MnO3 nanoparticles for magnetically driven DNA isolation

Type

journal article in Web of Science

Language

English

Original Abstract

Magnetic La0.75Sr0.25MnO3 nanoparticles possessing an approximately 20 nm thick silica shell (LSMO(0.25)SiO2) were characterised and tested for the isolation of PCR ready bacterial DNA. The results presented here show that the nanoparticles do not interfere in PCR. DNA was apparently reversibly adsorbed on their silica shell from the aqueous phase system (16 % PEG 6000, 2 M NaCl). The method proposed was used for DNA isolation from complex food samples (dairy products and probiotic food supplements). The isolated DNA was compatible with PCR. The main advantages of the nanoparticles tested for routine use were their high colloidal stability allowing a more precise dosage and therefore high reproducibility of DNA isolation.

Keywords

nanoparticles; lanthanum manganese perovskites; silica shell; DNA isolation; PCR

Authors

TRACHTOVÁ, Š.; KAMAN, O.; ŠPANOVÁ, A.; VEVERKA, P.; POLLERT, E.; RITTICH, B.

RIV year

2011

Released

1. 11. 2011

Publisher

John Wiley and Sons

Location

WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany

ISBN

1615-9306

Periodical

Journal of Separation Science

Year of study

34

Number

21

State

Federal Republic of Germany

Pages from

3077

Pages to

3082

Pages count

6

URL

http://onlinelibrary.wiley.com.ezproxy.lib.vutbr.cz/doi/10.1002/jssc.201100442/epdf

BibTex

@article{BUT73164,
  author="TRACHTOVÁ, Š. and KAMAN, O. and ŠPANOVÁ, A. and VEVERKA, P. and POLLERT, E. and RITTICH, B.",
  title="Silica coated La0.75Sr0.25MnO3 nanoparticles for magnetically driven DNA isolation",
  journal="Journal of Separation Science",
  year="2011",
  volume="34",
  number="21",
  pages="3077--3082",
  doi="10.1002/jssc.201100442",
  issn="1615-9306",
  url="http://onlinelibrary.wiley.com.ezproxy.lib.vutbr.cz/doi/10.1002/jssc.201100442/epdf"
}