Publication detail
Application of real-time PCR for evaluation of magnetic nanoparticles in molecular diagnostics
ŠPANOVÁ, A. TRACHTOVÁ, Š. RITTICH, B.
Original Title
Application of real-time PCR for evaluation of magnetic nanoparticles in molecular diagnostics
Type
abstract
Language
English
Original Abstract
In vitro amplification of nucleic acids using the polymerase chain reaction (PCR) has become a powerful diagnostic tool for the analysis of microorganisms. However, the sensitivity of PCR may be reduced when complex biological samples are studied, since various extracellular PCR inhibitors may be present. The problem can be resolved by means of various DNA isolation and purification methods. Magnetic particles can be successfully used for the isolation of DNA from various samples containing PCR inhibitors. Research has been focused on the development of innovative magnetic nanoparticles.
Keywords
magnetic particles, polymerase chain reaction, probiotic dairy products
Authors
ŠPANOVÁ, A.; TRACHTOVÁ, Š.; RITTICH, B.
Released
22. 5. 2011
Location
Pretoria - South Africa
ISBN
978-1-86854-946-7
Book
Book of Abstracts, 11th International Conference on Frontiers of Polymers and Advanced Materials, Pretoria
Pages count
1
BibTex
@misc{BUT72679,
author="Alena {Španová} and Štěpánka {Trachtová} and Bohuslav {Rittich}",
title="Application of real-time PCR for evaluation of magnetic nanoparticles in molecular diagnostics",
booktitle="Book of Abstracts, 11th International Conference on Frontiers of Polymers and Advanced Materials, Pretoria",
year="2011",
pages="1",
address="Pretoria - South Africa",
isbn="978-1-86854-946-7",
note="abstract"
}