Publication detail

Magnetic Microspheres for DNA Diagnostics and DNA Delivery

RITTICH, B. ŠPANOVÁ, A. TRACHTOVÁ, Š. HORÁK, D.

Original Title

Type

abstract

Language

English

Original Abstract

Magnetic nonporous hydrophilic poly(2-hydroxyethyl methacrylate-co-ethylene dimethacrylate) [P(HEMA-EDMA))], poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) [P(HEMA-GMA)], and poly(glycidyl methacrylate) [PGMA] microspheres were prepared by a single-step dispersion polymerisation in the presence of sterically or electrostatically stabilised colloidal magnetite or maghemite. Enzymes, such as RNase A, DNase I, proteinase K and Salmonella antibodies were immobilised on the microspheres and their applicability in molecular diagnostic was demonstrated. Heterogeneous lanthanide complexes of ethylenediaminetetraacetic and iminodiacetic acid immobilised on magnetic PGMA and P(HEMA-EDMA) microspheres, respectively, were used as catalysts of plasmid DNA cleavage. Monodisperse poly(N-isopropylacrylamide) microparticles were prepared by two methods, namely, ammonium persulfate-initiated precipitation polymerization in water and 2,2'-azobisisobutyronitrile-initiated and poly(hydrogenated isoprene-block-styrene)-stabilized dispersion polymerization in toluene/heptane. Microspheres were applied for reversible adsorption of DNA at different temperatures.

Keywords

nonporous magnetic hydrophilic microspheres; P(HEMA-co-EDMA); P(HEMA-co-GMA); PGMA; poly(N-isopropylacrylamide); enzyme immobilization; DNA isolation; PCR

Authors

RITTICH, B.; ŠPANOVÁ, A.; TRACHTOVÁ, Š.; HORÁK, D.

Released

30. 6. 2010

Publisher

VUTIUM

Location

Brno

ISBN

978-80-214-4112-5

Book

Book of abstract 6th International Conference on Materials Structure and Micromechanics of Fracture.

Pages from

151

Pages to

151

Pages count

BibTex

@misc{BUT72665,
  author="RITTICH, B. and ŠPANOVÁ, A. and TRACHTOVÁ, Š. and HORÁK, D.",
  title="Magnetic Microspheres for DNA Diagnostics and DNA Delivery",
  booktitle="Book of abstract 6th International Conference on Materials Structure and Micromechanics of Fracture.",
  year="2010",
  pages="151--151",
  publisher="VUTIUM",
  address="Brno",
  isbn="978-80-214-4112-5",
  note="abstract"
}