Publication detail

The application of PCR methods and gas chromatography for detection of specific non-pathogenic bacteria of the genus Clostridium

RITTICH, B., ŠPANOVÁ, A., URGEOVÁ, B., DUBSKÝ, H., KVASNIČKOVÁ, E., HAVLÍKOVÁ, Š.

Original Title

Type

abstract

Language

English

Original Abstract

The aim of this study was to select and investigate non-pathogenic bacterial strains of the genus Clostridium collected in the Dairy Research Institute. The strains were isolated from cheeses with late blowing disorders. First, a set of 44 strains was identified into the genus Clostridium using genus-specific PCR (Rekha et al., 2006). PCRs specific to the species Clostridium butyricum (Nakanishi et al., 2005) and Clostridium tyrobutyricum (Herman et al., 1995) were used for species identification. It was found that 7 and 12 strains belonged to the species C. butyricum or C. tyrobutyricum, respectively. Some strains were not identified into species yet. Strains encoding the H2-evolving hydrogenase (hydA) gene were also identified using PCR ( ). Formation of fatty acids (acetic, propionic, and butyric) was studied by gas chromatography in culture supernatants.

Keywords

Clostridium butyricum; Clostridium tyrobutyricum; hydrogenase (hydA); gas chromatography PCRs;

Authors

RITTICH, B., ŠPANOVÁ, A., URGEOVÁ, B., DUBSKÝ, H., KVASNIČKOVÁ, E., HAVLÍKOVÁ, Š.

Released

4. 12. 2009

Publisher

Formatex, Španělsko

Location

Lisabon, Portugalsko

Pages from

449

Pages to

449

Pages count

BibTex

@misc{BUT61055,
  author="Bohuslav {Rittich} and Alena {Španová} and Barbora {Gregušová}",
  title="The application of PCR methods and gas chromatography for detection of specific non-pathogenic bacteria of the genus Clostridium",
  year="2009",
  pages="449--449",
  publisher="Formatex, Španělsko",
  address="Lisabon, Portugalsko",
  note="abstract"
}