Publication detail

Production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by Cupriavidus necator from waste rapeseed oil using propanol as a precursor of 3-hydroxyvalerate

OBRUČA, S. MÁROVÁ, I. ŠNAJDAR, O. MRAVCOVÁ, L. SVOBODA, Z.

Original Title

Type

journal article - other

Language

English

Original Abstract

Waste rapeseed oil is a useful substrate for polyhydroxyalkanoates (PHA) production employing Cupriavidus necator H16. In fed-batch mode, we obtained biomass and PHA yields of 138 and 105 g l-1, respectively. Yield coefficient and volumetric productivity were 0.83 g PHA per g oil and 1.46 g l-1 h-1, espectively. Propanol at 1% (v/v) enhanced both PHA and biomass formation significantly and, furthermore, resulted in incorporation of 3-hydroxyvalerate units into PHA structure. Thus, propanol can be used as an effective precursor of 3-hydroxyvalarete for production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolymer. During the fed-batch cultivation, propanol concentration was maintained at 1% which resulted in 8% content of 3-hydroxyvalerate in copolymer.

Keywords

Cupriavidus necator, Polyhydroxyalkanoates, Propanol, Stress conditions, Waste edible oils

Authors

OBRUČA, S.; MÁROVÁ, I.; ŠNAJDAR, O.; MRAVCOVÁ, L.; SVOBODA, Z.

RIV year

2010

Released

6. 11. 2010

Publisher

Springer

Location

Amsterdam

ISBN

0141-5492

Periodical

Biotechnology Letters

Year of study

Number

State

Kingdom of the Netherlands

Pages from

1925

Pages to

1932

Pages count

BibTex

@article{BUT50131,
  author="Stanislav {Obruča} and Ivana {Márová} and Ondřej {Šnajdar} and Ludmila {Mravcová} and Zdeněk {Svoboda}",
  title="Production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by Cupriavidus necator from waste rapeseed oil using propanol as a precursor of 3-hydroxyvalerate",
  journal="Biotechnology Letters",
  year="2010",
  volume="32",
  number="12",
  pages="1925--1932",
  issn="0141-5492"
}