Publication detail

GROWTH CURVES OF MIXED THERMOPHILIC BACTERIA

BABÁK, L. BURDYCHOVÁ, R. DOHNAL, V.

Original Title

Type

journal article - other

Language

English

Original Abstract

The growth of mixed thermophilic bacteria culture (sludge of wastewater treatment Bystřice pod Hostýnem) was studied. Two different cultivation systems were used for monitoring and comparison of growth characteristics of above mentioned thermophilic culture. The first system consisted of batch cultivations in cuvettes with very little volumes (10-5 L) of cultivation media at 60 ?C. Samples were taken by autosampler in periodic intervals (24,4 min). Bacteria growth was there observed by means of HPLC with UV detection (600 nm). The second system was composed of batch cultivations in the laboratory fermentor BIOSTAT B ( B. Braun Biotech) at 60 ?C, too. The system was aerated (10 L.min-1) and stired by multiple turbo-stirrer (250 min-1). Samples were taken every two hours. Biomass concentration was detected by turbidimeter. Both cultivation ways were practised in several substrates with different C-sources (glucose, sacharose, lactose, maltose). Obtained growth curves were comparised. Furthermore, the biomass productivity values were determined.

Keywords

thermophilic, growth curve, HPLC/UV, bioreactor

Authors

BABÁK, L.; BURDYCHOVÁ, R.; DOHNAL, V.

RIV year

2008

Released

11. 9. 2008

Publisher

ČSCH

Location

Praha

ISBN

1803-2389

Periodical

Chemické listy (S)

Year of study

102

Number

State

Czech Republic

Pages from

583

Pages to

584

Pages count

BibTex

@article{BUT48885,
  author="Libor {Babák} and Radka {Burdychová} and Vlastimil {Dohnal}",
  title="GROWTH CURVES OF MIXED THERMOPHILIC BACTERIA",
  journal="Chemické listy (S)",
  year="2008",
  volume="102",
  number="15",
  pages="583--584",
  issn="1803-2389"
}