Publication detail

Functionalised magnetic microspheres with hydrophilic properties for molecular diagnostic applications

RITTICH B. ŠPANOVÁ A. HORÁK D.

Original Title

Type

journal article in Web of Science

Language

English

Original Abstract

Magnetic nonporous hydrophilic microspheres poly(2-hydroxyethyl methacrylate-co-ethylene dimethacrylate)-(P(HEMA-co-EDMA), poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate)-P(HEMA-co-GMA), and poly(glycidyl methacrylate)-PGMA were prepared by single-step dispersion polymerisation in the presence of sterically or electrostatically stabilised colloidal magnetite or maghemite. Several enzymes, such as RNase A, DNase I, proteinase K and Salmonella antibodies were immobilised on the microspheres and their applicability in degradation of bacterial RNA, chromosomal and plasmid DNAs, degradation of intracellular PCR inhibitors and immunomagnetic separation of Salmonella cells were demonstrated. Heterogeneous lanthanide complexes of ethylenediaminetetraacetic acid (EDTA) immobilised on insoluble PGMA support and iminodiacetic acid (IDA) immobilised on magnetic P(HEMA-co-EDMA) microspheres were used as catalysts of plasmid DNA cleavage.

Keywords

nonporous magnetic hydrophilic microspheres; P(HEMA-co-EDMA); P(HEMA-co-GMA); PGMA; enzyme immobilization; PCR-ready DNA; amplification methods

Authors

RITTICH B.; ŠPANOVÁ A.; HORÁK D.

RIV year

2009

Released

10. 5. 2009

Publisher

Elsevier

Location

Amsterdam Holandsko

ISBN

0963-9969

Periodical

FOOD RESEARCH INTERNATIONAL

Year of study

Number

State

United States of America

Pages from

493

Pages to

498

Pages count

BibTex

@article{BUT48518,
  author="RITTICH B. and ŠPANOVÁ A. and HORÁK D.",
  title="Functionalised magnetic microspheres with hydrophilic properties for molecular diagnostic applications",
  journal="FOOD RESEARCH INTERNATIONAL",
  year="2009",
  volume="42",
  number="4",
  pages="493--498",
  issn="0963-9969"
}