Publication detail

Stability of collagen scaffold implants for animals with iatrogenic articular cartilage defoects

JANČÁŘ, J. VOJTOVÁ, L. NEČAS, A. SRNEC, R. URBANOVÁ, L. CRHA, M.

Original Title

Stability of collagen scaffold implants for animals with iatrogenic articular cartilage defoects

Type

journal article - other

Language

English

Original Abstract

Synthesis and characterization of biodegradable hydrogels based on collagen modified by addition of synthetic biodegradable copolymer intended for preparation of porous scaffolds for mesenchymal stem cells used for possible implantation to animals with articular surface defects was investigated. The synthetic biodegradable tri-block copolymer used was the block copolymer of polyethylene glycol (PEG), polylactic acid (PLA), polyglycolic acid (PGA) (PEG-PLGA) endcapped with itaconic acid (ITA). The water-soluble carbodiimide and N-hydroxysuccimide system (EDC-NHS) was chosen as the cross-linking agent used to control the rate of hydrogel resorption. Dependence of the physical properties of the prepared hydrogels on the concentration of the EDC-NHS cross-linker, reaction time and concentration of PEG-PLGA-ITA copolymer was examined. Swelling behaviour, thermal stability, surface morphology and degradation rate were also characterized. Based on the obtained results, it can be concluded that increase in concentration of the cross-linking agent, as well as prolonged cross-linking time and increased amount of synthetic copolymer lead to enhanced thermal stability of the gels together with a reduced swelling ratio and degradation rate in saline. The resorption rate of these gels used in preparation of cartilage scaffolds can be controlled over a wide time interval by varying the collagen/(PEG-PLGA-ITA) blend composition or the conditions of the cross-linking reaction.

Keywords

collagen, PEG, PLA, PGA, EDC, cross-linking, scaffold

Authors

JANČÁŘ, J.; VOJTOVÁ, L.; NEČAS, A.; SRNEC, R.; URBANOVÁ, L.; CRHA, M.

RIV year

2009

Released

15. 12. 2009

Publisher

University of Veterinary and Pharmaceutical Sciences in Brno

Location

Brno

ISBN

0001-7213

Periodical

ACTA VETERINARIA BRNO

Year of study

78

Number

4

State

Czech Republic

Pages from

643

Pages to

648

Pages count

6

URL

https://actavet.vfu.cz/78/4/0643/

Full text in the Digital Library

http://hdl.handle.net/11012/137207

BibTex

@article{BUT47810,
  author="Josef {Jančář} and Lucy {Vojtová} and Alois {Nečas} and Robert {Srnec} and Lucie {Urbanová} and Michal {Crha}",
  title="Stability of collagen scaffold implants for animals with iatrogenic articular cartilage defoects",
  journal="ACTA VETERINARIA BRNO",
  year="2009",
  volume="78",
  number="4",
  pages="643--648",
  doi="10.2754/avb200978040643",
  issn="0001-7213",
  url="https://actavet.vfu.cz/78/4/0643/"
}