Publication detail

Exploration on the separability of thaumatin by capillary electrophoresis

VESPALCOVÁ, M. GREGOROVÁ, D.

Original Title

Type

journal article - other

Language

English

Original Abstract

Electrophoretic behaviour of highly basic protein thaumatin was explored in strongly acid (pH 2) and mildly acid (pH 4.5) separation systems using both bare and coated fused silica capillaries. Separation selectivity for thaumatin I, thaumatin II and for other sample constituents was nor sufficient for their baseline separation at pH 2 with uncoated capillary because of separation efficiency, which was markedly lower than common in electrophoretic separations of proteins. Separation selectivity higher up to one order of magnitude has been reached at pH 4.5. Pronounced asymmetry of zones, which deteriorated resolution at this pH, was effectively suppressed by the coating of the capillary wall with a polymer. Electrochromatographic separation systems based on capillaries coated with the layer of either cationic or hydrophilic uncharged polymer appear to be promising for the development of methods for thaumatin analysis.

Keywords

Thaumatin; CE; Basic protein; Separation; Electrochromatography

Authors

VESPALCOVÁ, M.; GREGOROVÁ, D.

Released

1. 8. 2003

Publisher

Wiley-VCH

Location

Weinheim

ISBN

1615-9306

Periodical

Journal of Separation Science

Year of study

Number

State

Federal Republic of Germany

Pages from

727

Pages to

734

Pages count

BibTex

@article{BUT46009,
  author="Milena {Vespalcová} and Daniela {Gregorová}",
  title="Exploration on the separability of thaumatin by capillary electrophoresis",
  journal="Journal of Separation Science",
  year="2003",
  volume="26",
  number="8",
  pages="727--734",
  issn="1615-9306"
}