Publication detail

Findings and indication in the PAGE- analysis of sweet protein thaumatin.

VESPALCOVÁ, M. HOLIŠOVÁ, R. VRÁNOVÁ, D. TŮMA, Z.

Original Title

Type

journal article - other

Language

English

Original Abstract

Thaumatin (E 957) is a low-caloric alternative sweetener produced from fruits of the tropical plant Thaumatococcus danielli Benth.. It is the sweetest known substance, is approximately 3000 times sweeter than sucrose and acts as a taste modifier, too. Chemically, thaumatin is a basic protein, reportedly of extremely high pI = 11.7-12.0 [1] and of relative molecular mass 22 kDa . Commercially available thaumatin preparations consists of two main fractions, thaumatin I and thaumatin II, that differ only by five aminoacids in their chains, and several minor proteinic admixtures. Complete separation of constituents in commercially available thaumatins is very difficult. Well documented analytical technique for the purpose is missing yet. We decided therefore to start systematic research on the thaumatin analysis by exploration on analytical potential of PAGE techniques whose utilization for the purpose is the most frequently reported. Experiments at both non-denaturating and denaturating conditions show that neither thaumatin pI derived from flat bed gel analyses nor efficacy of the SDS-PAGE method for thaumatin, respectively, are reliable. It casts doubt on the capability of flat bed gel analyses to give credible analytical results for thaumatin as the analyte.

Keywords

Thaumatin, PAGE-analysis, SDS-PAGE, basic protein,

Authors

VESPALCOVÁ, M.; HOLIŠOVÁ, R.; VRÁNOVÁ, D.; TŮMA, Z.

RIV year

2005

Released

25. 8. 2005

Publisher

Česká společnost chemická

Location

Brno

ISBN

0009-2770

Periodical

Chemické listy

Year of study

Number

State

Czech Republic

Pages from

362

Pages to

364

Pages count

BibTex

@article{BUT42745,
  author="Milena {Vespalcová} and Radana {Holišová} and Dana {Vránová} and Zdeněk {Tůma}",
  title="Findings and indication in the PAGE- analysis of sweet protein thaumatin.",
  journal="Chemické listy",
  year="2005",
  volume="99",
  number="S",
  pages="362--364",
  issn="0009-2770"
}