Publication detail

The role of glycerol in manufacturing freeze-dried chitosan and cellulose foams for mechanically stable scaffolds in skin tissue engineering

VERČIMÁKOVÁ, K. KARBOWNICZEK, J. SEDLÁŘ, M. STACHEWICZ, U. VOJTOVÁ, L.

Original Title

The role of glycerol in manufacturing freeze-dried chitosan and cellulose foams for mechanically stable scaffolds in skin tissue engineering

Type

journal article in Web of Science

Language

English

Original Abstract

Various strategies have extensively explored enhancing the physical and biological properties of chitosan and cellulose scaffolds for skin tissue engineering. This study presents a straightforward method involving the addition of glycerol into highly porous structures of two polysaccharide complexes: chitosan/carboxymethyl cellulose (Chit/CMC) and chitosan/oxidized cellulose (Chit/OC); during a one-step freeze-drying process. Adding glycerol, especially to Chit/CMC, significantly increased stability, prevented degradation, and improved mechanical strength by nearly 50%. Importantly, after 21 days of incubation in enzymatic medium Chit/CMC scaffold has almost completely decomposed, while foams reinforced with glycerol exhibited only 40% mass loss. It is possible due to differences in multivalent cations and polymer chain contraction, resulting in varied hydrogen bonding and, consequently, distinct physicochemical outcomes. Additionally, the scaffolds with glycerol improved the cellular activities resulting in over 40% higher proliferation of fibroblast after 21 days of incubation. It was achieved by imparting water resistance to the highly absorbent material and aiding in achieving a balance between hydrophilic and hydrophobic properties. This study clearly indicates the possible elimination of additional crosslinkers and multiple fabrication steps that can reduce the cost of scaffold production for skin tissue engineering applications while tailoring mechanical strength and degradation.

Keywords

Chitosan; Cellulose; Ionic complex; Glycerol; Tissue engineering

Authors

VERČIMÁKOVÁ, K.; KARBOWNICZEK, J.; SEDLÁŘ, M.; STACHEWICZ, U.; VOJTOVÁ, L.

Released

2. 7. 2024

Publisher

ELSEVIER

Location

AMSTERDAM

ISBN

1879-0003

Periodical

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Year of study

275

Number

Volume 275, Part

State

Kingdom of the Netherlands

Pages from

1

Pages to

12

Pages count

12

URL

https://www.sciencedirect.com/science/article/pii/S0141813024044076#f0005

BibTex

@article{BUT189828,
  author="Katarína {Verčimáková} and Joanna {Karbowniczek} and Marian {Sedlář} and Urszula {Stachewicz} and Lucy {Vojtová}",
  title="The role of glycerol in manufacturing freeze-dried chitosan and cellulose foams for mechanically stable scaffolds in skin tissue engineering",
  journal="INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES",
  year="2024",
  volume="275",
  number="Volume 275, Part",
  pages="1--12",
  doi="10.1016/j.ijbiomac.2024.133602",
  issn="1879-0003",
  url="https://www.sciencedirect.com/science/article/pii/S0141813024044076#f0005"
}