Publication detail

The effect of surface roughness and wettability on the adhesion and proliferation of Saos-2 cells seeded on 3D printed poly(3-hydroxybutyrate)/polylactide (PHB/PLA) surfaces

MELČOVÁ, V. KROBOT, Š. ŠINDELÁŘ, J. ŠEBOVÁ, E. KLUSÁČEK RAMPICHOVÁ, M. PŘIKRYL, R.

Original Title

The effect of surface roughness and wettability on the adhesion and proliferation of Saos-2 cells seeded on 3D printed poly(3-hydroxybutyrate)/polylactide (PHB/PLA) surfaces

Type

journal article in Web of Science

Language

English

Original Abstract

The aim of this work is to explore the effect of surface characteristics of the 3D printed scaffolds on their suitability for Saos-2 cells growth. Two promising materials for bone tissue engineering were examined, both containing poly(3-hydroxybutyrate)/poly(d,l-lactide) plasticized blend and one of them filled with bioactive tricalcium phosphate. The surface free energy (SFE) of samples was determined by contact angle measurement with four liquids. For non-filled sample the water contact angle (WCA) was 74° and its SFE was 40 mN∙m−1. The composite sample exhibited substantially decreased WCA, 61°. Moreover, the addition of the tricalcium phosphate caused doubling of the polar component of the SFE which increased by a total of 13%. 3D printed surfaces prepared by fused deposition modeling method showed a profound increase of WCA due to the increase in their surface roughness which was analyzed by confocal microscopy. The change in wetting properties strongly affected the behavior of Saos-2 cells on printed surfaces. The number of cells as measured by DNA quantification linearly decreased with increasing surface WCA. The highest number of cells was observed on the pressed samples and 3D printed surfaces made of simple lines and a grid with 50 μm gap between lines. The favored surfaces exhibit WCA under 80° which is important information for the future design of the scaffolds.

Keywords

Poly(3-hydroxybutyrate); polylactide; 3D printing; water contact angle; surface energy; surface roughness

Authors

MELČOVÁ, V.; KROBOT, Š.; ŠINDELÁŘ, J.; ŠEBOVÁ, E.; KLUSÁČEK RAMPICHOVÁ, M.; PŘIKRYL, R.

Released

1. 8. 2024

Publisher

Elsevier

ISBN

2666-8459

Periodical

Results in Surfaces and Interfaces

Year of study

16

Number

August

State

Kingdom of the Netherlands

Pages from

1

Pages to

18

Pages count

18

URL

https://www.sciencedirect.com/science/article/pii/S2666845924000916?via%3Dihub

Full text in the Digital Library

http://hdl.handle.net/11012/249739

BibTex

@article{BUT189307,
  author="Veronika {Melčová} and Štěpán {Krobot} and Jan {Šindelář} and Eva {Šebová} and Michala {Klusáček Rampichová} and Radek {Přikryl}",
  title="The effect of surface roughness and wettability on the adhesion and proliferation of Saos-2 cells seeded on 3D printed poly(3-hydroxybutyrate)/polylactide (PHB/PLA) surfaces",
  journal="Results in Surfaces and Interfaces",
  year="2024",
  volume="16",
  number="August",
  pages="1--18",
  doi="10.1016/j.rsurfi.2024.100271",
  issn="2666-8459",
  url="https://www.sciencedirect.com/science/article/pii/S2666845924000916?via%3Dihub"
}