CULTIVATION OF CAROTENOGENIC YEAST ON VARIOUS FOOD INDUSTRIAL WASTE SUBSTRATES

presentation, poster

English

Carotenogenic yeasts are a group of yeasts that produce orange to red pigments in the form of carotenoids. They are also known for their ability to produce a significant amount of other bioactive substances, including ubiquinone, ergosterol, betaglucans, proteins and lipids. Their great biotechnological potential lies in the ability of utilizing many waste materials to produce biomass enriched by the bioactive substances under the biorefinery concept. The adaptability of carotenogenic yeasts allows them to use different waste substrates for their growth, e.g. oils and fats, chicken feather hydrolysate, lignocellulose materials and many others. Specifically, this study focuses on combined utilization of whey, waste poultry fat and waste frying oil as nutrition sources. In this study, the yeast strains Rhodotorula toruloides and Cystofilobasidium macerans were studied. Batch cultivations were carried out in artificial media with the addition of waste substrates at different C/N ratios. In these experiments, the best yield was achieved by the strain Cystofilobasidium macerans on waste whey with the biomass yield 21.65 g/l. The strain Rhodotorula toruloides achieved an increased yield of biomass (14.35 g/l) on glycerol, which can serve as a model for waste glycerol obtained from biofuel production. The main goal of future research is to test also other red yeast strains such as Phaffia sp. and Sporidiobolus sp. in the presence of waste substrates and to scale-up these cultivations to the laboratory bioreactor.

Carotenogenic yeasts, waste poultry fat, waste frying oil, waste substrates

HOLUB, J.; CHUJANOV, O.; ŠPAČKOVÁ, D.; ŠIMANSKÝ, S.; OBRAČAJ, J.; MÁROVÁ, I.

15. 4. 2024

AMCA, spol. s r.o.

Vyšehradská 320/49 128 00 Prague 2 Czech Republic

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https://www.icct.cz/en/Amca-ICCT/media/content/2024/documents/Book_of_abstracts_-ICCT2024.pdf