Publication detail

High resolution single cell LA-ICP-MS 2D imaging

Kristýna Bilavčíková, Michaela Vašinová Galiová, Roman Hrstka, Jiří Pinkas, Jindřich Karban

Original Title

Type

conference proceedings

Language

English

Original Abstract

This work focuses on the use of LA-ICP-MS for 2D-imaging of A549 cell line, a lung cancer epithelial cell line, injected with a newly synthesized unique potential agent based on ruthenium. A fundamental study of the laser ablation working parameters was performed, providing a detailed imaging of the distribution of the 101Ru+ isotope with a resolution of 2 µm. In addition, trends in influx and efflux and agent distribution were monitored as a function of cell harvest time at 6, 12 and 24 hours after drug application. It was discovered that most of the agent was found in the cell samples taken at 12 hours after treatment and it was predicted that there is probably preferential accumulation of the drug in the nuclei of the cells.

Keywords

LA-ICP-MS; 2D imaging; single cell analysis; high-resolution

Authors

Kristýna Bilavčíková, Michaela Vašinová Galiová, Roman Hrstka, Jiří Pinkas, Jindřich Karban

Released

27. 5. 2024

ISBN

978-80-88195-54-2

Book

CSSC & MSMS 2024 book of abstracts

BibTex

@proceedings{BUT189052,
  editor="Kristýna {Bilavčíková} and Michaela {Vašinová Galiová}",
  title="High resolution single cell LA-ICP-MS 2D imaging",
  year="2024",
  isbn="978-80-88195-54-2"
}