Advanced 3D collagen-based scaffolds modified with pro-angiogenic molecule for wound healing

IZSÁK, D. PAVLIŇÁKOVÁ, V. FOHLEROVÁ, Z. SZOTKOWSKÁ, T. BUCHTOVÁ, M. VOJTOVÁ, L.

Original Title

Type

abstract

Language

English

Original Abstract

Skin serves as a protective barrier for the human body against toxins and microbes in the environment. The presence of biomaterials at a wound site plays a crucial role in influencing the production of the skin's extracellular matrix (ECM). This research specifically delves into protein-based biomaterials designed to mimic the ECM and regulate biomechanical processes at the cellular level, including cell migration, proliferation, differentiation as well as neovascularization. In this study, porous 3D collagen scaffolds were prepared by using freeze-drying method. To investigate the effect of additives the scaffolds were modified with chitosan following with crosslinking using carbodiimide crosslinking agents. Subsequently, varying concentrations of a pro-angiogenic bioactive substance were incorporated into the scaffolds to enhance the production of vascular endothelial growth factor in fibroblast cells and to improve dermal wound healing. Physicochemical characterization of the prepared carriers involved techniques such as scanning electron microscopy to assess morphology and pore size. The release of pro-angiogenic molecules from the scaffolds was monitored through UV-VIS analysis. Furthermore, cytotoxicity assessments were conducted in-vitro using mouse fibroblasts, while neovascularization studies were performed in-ovo using chicken embryos. This comprehensive approach not only elucidates the potential of protein-based biomaterials in wound healing but also highlights the importance of tailored scaffold design for promoting effective tissue regeneration. This work was supported by the Ministry of Health of the Czech Republic under project No. NU22-08–00454 as well as and the European Fund for Regional Development within the project No. CZ.02.01.01/00/22_008/0004562. CzechNanoLab project LM2023051 funded by MEYS CR is gratefully acknowledged for the financial support of the measurements at CEITEC Nano Research Infrastructure.

Keywords

Dermis, wound healing, vascularization, release, collagen, chitosan, scaffold, regenerative medicine

Authors

IZSÁK, D.; PAVLIŇÁKOVÁ, V.; FOHLEROVÁ, Z.; SZOTKOWSKÁ, T.; BUCHTOVÁ, M.; VOJTOVÁ, L.

Released

25. 4. 2024

Location

Praha

ISBN

978-80-01-07293-6

Book

Bioimplantologie a regenerativní medicína 2024

Edition number

Pages from

Pages to

Pages count

URL

https://921c88450d.clvaw-cdnwnd.com/17e12706bb26b88046060a8921d16f31/200000499-73ed673ed8/bioimplantologie_2024_sbornik.pdf?ph=921c88450d

BibTex

@misc{BUT188616,
  author="Dávid {Izsák} and Veronika {Pavliňáková} and Zdenka {Fohlerová} and Tereza {Szotkowská} and Marcela {Buchtová} and Lucy {Vojtová}",
  title="Advanced 3D collagen-based scaffolds modified with pro-angiogenic molecule for wound healing",
  booktitle="Bioimplantologie a regenerativní medicína 2024",
  year="2024",
  edition="1.",
  pages="35--35",
  address="Praha",
  isbn="978-80-01-07293-6",
  url="https://921c88450d.clvaw-cdnwnd.com/17e12706bb26b88046060a8921d16f31/200000499-73ed673ed8/bioimplantologie_2024_sbornik.pdf?ph=921c88450d",
  note="abstract"
}