Publication detail

Bioinformatics study of the third generation sequencing platforms applied on a thermophile

UMAIR, M. ŘEHÁKOVÁ, V. BUCHTÍKOVÁ, I. BEZDÍČEK, M. OBRUČA, S. SEDLÁŘ, K.

Original Title

Type

article in a collection out of WoS and Scopus

Language

English

Original Abstract

This study compares the efficiency of Pacific Biosciences technology (PacBio) and Oxford Nanopore Technology (ONT) in sequencing, assembling, and annotating the Aneurinibacillus sp AFn2 bacterium. We aim to evaluate the performance based on contiguity, depth, and functional annotation of the resulting genome. Using ONT we generated 152,047 long reads assembling into 2 contigs with total base count of 0.4 billion which provided us efficient assembly while PacBio produced 139,701 reads, assembling into 21 contigs with a total base count of 1.4 billion. Functional annotation revealed differences in the number of coding sequences, with PacBio detecting more comprehensive gene sets than ONT. The comparative analysis done in this research shows the strengths and limitations of both the platforms, with ONT providing higher assembly contiguity and PacBio offering greater detail in genetic content. We aim to offer insights of both the sequencing technologies, guiding researchers in selecting the appropriate technology

Keywords

Aneurinibacillus, Nanopore, PacBio, Sequencing, Genome Assembly

Authors

UMAIR, M.; ŘEHÁKOVÁ, V.; BUCHTÍKOVÁ, I.; BEZDÍČEK, M.; OBRUČA, S.; SEDLÁŘ, K.

Released

24. 4. 2024

ISBN

978-80-214-6231-1

Book

Proceedings I of the 30 th Conference STUDENT EEICT 2024

Edition

Pages count

BibTex

@inproceedings{BUT188508,
  author="Mohammad {Umair} and Veronika {Řeháková} and Iva {Buchtíková} and Matěj {Bezdíček} and Stanislav {Obruča} and Karel {Sedlář}",
  title="Bioinformatics study of the third generation sequencing platforms applied on a thermophile",
  booktitle="Proceedings I of the 30 th Conference STUDENT EEICT 2024",
  year="2024",
  series="1",
  pages="5",
  isbn="978-80-214-6231-1"
}