The influence of different wheat bran pretreatment methods on the yield and purity of isolated ferulic acid after alkaline hydrolysis

abstract

English

Ferulic acid (FA) is a highly valued bioactive substance known for its significant antioxidant, antimicrobial, anticancer, antiallergic, hepatoprotective, anti-inflammatory, and antiviral effects. Due to these properties, the use of FA in the food, pharmaceutical, and cosmetic industries is growing. Recently, substantial efforts have been made to isolate this compound from residual biomass with the aim of overcoming production by petrochemical means. One of the interesting materials containing ferulic acid available in large quantities is wheat bran [1;2]. According to FAOSTAT, the world's processing of wheat produces more than 120 million metric tons of wheat bran [3]. In this study, the effect of applying different pre-treatment methods to wheat bran before alkaline hydrolysis and the isolation of FA was tested. A single-step pre-treatment was examined using hot water or 0.05 M NaOH, as well as a two-step pre-treatment, involving both of these steps sequentially. The yield and purity of ferulic acid obtained through these procedures were compared with the process using alkaline hydrolysis without bran pre-treatment. The alkaline hydrolysis was performed using 0.2 M NaOH at a temperature of 50°C with 2 hours of stirring. For the isolation of ferulic acid from the hydrolysate, adsorption and desorption using XAD-16 resin were performed [4]. The yield of FA using alkaline hydrolysis without bran pre-treatment was 1.5 mg/g; however, the purity of the isolated FA was relatively low (12%). By pretreating wheat bran with hot water, a lower yield of FA was achieved (0.99 mg/g); however, the purity was slightly improved (16%). When 0.05 M NaOH was used for the pretreatment of wheat bran, the yield of FA was 0.87 mg/g, and the purity increased to an average of 21%. The simultaneous combination of both pretreatment methods did not lead to further improvements in the yield or purity of FA. Tests are currently underway using hydrogen peroxide in the process of FA extraction, and preliminary results show that satisfactory yields and purities of FA over 40% can be achieved using this process.

Biorafinery, adsorption, ferulic acid, wheat bean, XAD-16, HPLC

NÁBĚLEK, J.; DIVIŠ, P.; POŘÍZKA, J.

30. 11. 2023

Vysoké učení technické v Brně, Fakulta chemická

Purkyňova 464/118, 612 00 Brno

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https://www.fch.vut.cz/vav/konference/sok/vystupy/sbornik-abstraktu-2023-na-web-pdf-p251584