Publication detail

Liposomes Affect Protein Release and Stability of ITA-Modified PLGA-PEG-PLGA Hydrogel Carriers for Controlled Drug Delivery

KADLECOVÁ, Z. SEVRIUGINA, V. LYSÁKOVÁ, K. RYCHETSKÝ, M. CHAMRADOVÁ, I. VOJTOVÁ, L.

Original Title

Liposomes Affect Protein Release and Stability of ITA-Modified PLGA-PEG-PLGA Hydrogel Carriers for Controlled Drug Delivery

Type

journal article in Web of Science

Language

English

Original Abstract

Fat grafting, a key regenerative medicine technique, often requires repeat procedures due to high-fat reabsorption and volume loss. Addressing this, a novel drug delivery system uniquely combines a thermosensitive, FDA-approved hydrogel (itaconic acid-modified PLGA-PEG-PLGA copolymer) with FGF2-STAB, a stable fibroblast growth factor 2 with a 21-day stability, far exceeding a few hours of wild-type FGF2's stability. Additionally, the growth factor was encapsulated in "green" liposomes prepared via the Mozafari method, ensuring pH protection. The system, characterized by first-order FGF2-STAB release, employs green chemistry for biocompatibility, bioactivity, and eco-friendliness. The liposomes, with diameters of 85.73 +/- 3.85 nm and 68.6 +/- 2.2% encapsulation efficiency, allowed controlled FGF2-STAB release from the hydrogel compared to the unencapsulated FGF2-STAB. Yet, the protein compromised the carrier's hydrolytic stability. Prior tests were conducted on model proteins human albumin (efficiency 80.8 +/- 3.2%) and lysozyme (efficiency 81.0 +/- 2.7%). This injectable thermosensitive system could advance reconstructive medicine and cosmetic procedures.

Keywords

Drug delivery, PLGA-PEG-PLGA copolymer, itaconic acid, FGF2-STAB®, Mozafari method, liposomes

Authors

KADLECOVÁ, Z.; SEVRIUGINA, V.; LYSÁKOVÁ, K.; RYCHETSKÝ, M.; CHAMRADOVÁ, I.; VOJTOVÁ, L.

Released

22. 12. 2023

Publisher

AMER CHEMICAL SOC

Location

WASHINGTON

ISBN

1525-7797

Periodical

BIOMACROMOLECULES

Year of study

25

Number

1

State

United States of America

Pages from

67

Pages to

76

Pages count

10

URL

https://pubs.acs.org/doi/epdf/10.1021/acs.biomac.3c00736

Full text in the Digital Library

http://hdl.handle.net/11012/245202

BibTex

@article{BUT187107,
  author="Zuzana {Kadlecová} and Veronika {Sevriugina} and Klára {Tušlová} and Matěj {Rychetský} and Ivana {Chamradová} and Lucy {Vojtová}",
  title="Liposomes Affect Protein Release and Stability of ITA-Modified PLGA-PEG-PLGA Hydrogel Carriers for Controlled Drug Delivery",
  journal="BIOMACROMOLECULES",
  year="2023",
  volume="25",
  number="1",
  pages="67--76",
  doi="10.1021/acs.biomac.3c00736",
  issn="1525-7797",
  url="https://pubs.acs.org/doi/epdf/10.1021/acs.biomac.3c00736"
}