Publication detail

3D Collagen-based Scaffolds Loaded with Bioactive Molecules for Regenerative Medicine

IZSÁK, D. PAVLIŇÁKOVÁ, V. FOHLEROVÁ, Z. SZOTKOWSKÁ, T. BUCHTOVÁ, M. VOJTOVÁ, L.

Original Title

Type

conference proceedings

Language

English

Original Abstract

The skin is the largest organ of the body. The skin has an immune system that protects the body from toxins and infections, and attempts to prevent autoimmunity, in addition to being a physical barrier against the external environment. Biomaterials used in skin injury have a significant effect on the production of skin extracellular matrix (ECM). This work focuses on protein-based biomaterials that have the ability to mimic the ECM and also help control biomechanical processes at the cellular level, such as cell migration, cell proliferation, and cell differentiation. Collagen is a widely used biopolymer in regenerative medicine and tissue engineering due to its biocompatibility, low antigenicity, biodegradability, and cross-linking ability. It is used in a broad range of medical applications including pharmaceuticals, cosmetics, drug delivery, cell regeneration, and wound dressings. Additionally, its high hydrophilicity promotes moist healing, which is crucial for effective epithelium restoration. Wound healing is a multicellular process that relies on a complexly regulated signaling network. In this study, porous 3D collagen scaffolds were prepared by the freeze-drying method. To investigate the effect of additives the scaffolds were modified with antimicrobial chitosan. The hydrolytic stability of prepared scaffolds was supported by crosslinking process. Subsequently, bioactive molecules were incorporated into the scaffolds, and its release was monitored using UV-VIS analysis. The prepared carriers were characterized through various methods and analysis, including scanning electron microscopy to examine morphology and porosity, as well as FTIR, swelling and hydrolytic degradation. Cytotoxicity of the prepared samples was evaluated by in vitro testing on 3T3 fibroblast cells and the vasculogenic potential was evaluated via in ovo chick chorioallantoic membrane (CAM) assay. Newly prepared scaffolds may have a potential application in surgery and regenerative medicine of soft tissues.

Keywords

Wound dressing, scaffold, collagen, chitosan, dermis, regenerative medicine, drug release, bioactive molecule

Authors

IZSÁK, D.; PAVLIŇÁKOVÁ, V.; FOHLEROVÁ, Z.; SZOTKOWSKÁ, T.; BUCHTOVÁ, M.; VOJTOVÁ, L.

Released

30. 11. 2023

Publisher

Vysoké učení technické v Brně, Fakulta chemická, Purkyňova 464/118, 612 00 Brno

Location

Brno

ISBN

978-80-214-6204-5

Book

Studentská odborná konference Chemie je život 2023 Sborník abstraktů

Edition number

první

Pages from

Pages to

Pages count

URL

https://www.fch.vut.cz/vav/konference/sok/vystupy/sbornik-abstraktu-2023-na-web-pdf-p251584?aid_redir=1

BibTex

@proceedings{BUT186974,
  editor="Dávid {Izsák} and Veronika {Pavliňáková} and Zdenka {Fohlerová} and Tereza {Szotkowská} and Marcela {Buchtová} and Lucy {Vojtová}",
  title="3D Collagen-based Scaffolds Loaded with Bioactive Molecules for Regenerative Medicine",
  year="2023",
  number="první",
  pages="79--79",
  publisher="Vysoké učení technické v Brně, Fakulta chemická, Purkyňova 464/118, 612 00 Brno",
  address="Brno",
  isbn="978-80-214-6204-5",
  url="https://www.fch.vut.cz/vav/konference/sok/vystupy/sbornik-abstraktu-2023-na-web-pdf-p251584?aid_redir=1"
}