Publication detail

The presence of a G-quadruplex prone sequence upstream of a minimal promoter increases transcriptional activity in the yeast Saccharomyces cerevisiae

KRATOCHVILOVÁ, L. VOJSOVIČ, M. VALKOVÁ, N. ŠISLEROVÁ, L. EL RASHED, Z. INGA, A. MONTI, P. BRÁZDA, V.

Original Title

The presence of a G-quadruplex prone sequence upstream of a minimal promoter increases transcriptional activity in the yeast Saccharomyces cerevisiae

Type

journal article in Web of Science

Language

English

Original Abstract

Non-canonical secondary structures in DNA are increasingly being revealed as critical players in DNA metabolism, including modulating the accessibility and activity of promoters. These structures comprise the so-called G-quadruplexes (G4s) that are formed from sequences rich in guanine bases. Using a well-defined transcriptional reporter system, we sought to systematically investigate the impact of the presence of G4 structures on transcription in yeast Saccharomyces cerevisiae. To this aim, different G4 prone sequences were modeled to vary the chance of intramolecular G4 formation, analyzed in vitro by Thioflavin T binding test and circular dichroism and then placed at the yeast ADE2 locus on chromosome XV, downstream and adjacent to a P53 response element (RE) and upstream from a minimal CYC1 promoter and Luciferase 1 (LUC1) reporter gene in isogenic strains. While the minimal CYC1 promoter provides basal reporter activity, the P53 RE enables LUC1 transactivation under the control of P53 family proteins expressed under the inducible GAL1 promoter. Thus, the impact of the different G4 prone sequences on both basal and P53 family protein-dependent expression was measured after shifting cells onto galactose containing medium. The results showed that the presence of G4 prone sequences upstream of a yeast minimal promoter increased its basal activity proportionally to their potential to form intramolecular G4 structures; consequently, this feature, when present near the target binding site of P53 family transcription factors, can be exploited to regulate the transcriptional activity of P53, P63 and P73 proteins.

Keywords

G-quadruplex; p53; transcriptional activity; yeast

Authors

KRATOCHVILOVÁ, L.; VOJSOVIČ, M.; VALKOVÁ, N.; ŠISLEROVÁ, L.; EL RASHED, Z.; INGA, A.; MONTI, P.; BRÁZDA, V.

Released

20. 12. 2023

Publisher

PORTLAND PRESS LTD

Location

LONDON

ISBN

0144-8463

Periodical

BIOSCIENCE REPORTS

Year of study

43

Number

12

State

United States of America

Pages count

14

URL

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10730334/

BibTex

@article{BUT185731,
  author="KRATOCHVILOVÁ, L. and VOJSOVIČ, M. and VALKOVÁ, N. and ŠISLEROVÁ, L. and EL RASHED, Z. and INGA, A. and MONTI, P. and BRÁZDA, V.",
  title="The presence of a G-quadruplex prone sequence upstream of a minimal promoter increases transcriptional activity in the yeast Saccharomyces cerevisiae",
  journal="BIOSCIENCE REPORTS",
  year="2023",
  volume="43",
  number="12",
  pages="14",
  doi="10.1042/BSR20231348",
  issn="0144-8463",
  url="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10730334/"
}