Publication detail

Synergistic antibacterial action of the iron complex and ampicillin against Staphylococcus aureus

KOŠARIŠŤANOVÁ, L. ŘÍHÁČEK, M. SUCHÁ, F. MILOSAVLJEVIĆ, V. ŠVEC, P. DORAZILOVÁ, J. VOJTOVÁ, L. ANTAL, P. KOPEL, P. PATOČKA, Z. ADAM, V. ŽŮREK, L. DOLEŽELÍKOVÁ, K.

Original Title

Type

journal article in Web of Science

Language

English

Original Abstract

Resistance to antibiotics among bacteria of clinical importance, including Staphylococcus aureus, is a serious problem worldwide and the search for alternatives is needed. Some metal complexes have antibacterial properties and when combined with antibiotics, they may increase bacterial sensitivity to antimicrobials. In this study, we synthesized the iron complex and tested it in combination with ampicillin against S. aureus. An iron complex was synthesized and characterized using spectroscopy methods. Confirmation of the synergistic effect between the iron complex (Fe16) and ampicilin (AMP) was performed using zeta-potential, infrared spectra and FICI index calculated from the minimum inhibitory concentration (MIC) from the checkerboard assay. Cytotoxic properties of combination Fe16 + AMP was evaluated on eukaryotic cell line. Impact of combination Fe16 + AMP on chosen genes of S. aureus were performed by Quantitative Real-Time PCR. The MIC of Fe16 + AMP was significantly lower than that of AMP and Fe16 alone. Furthermore, the infrared spectroscopy revealed the change in the zeta-potential of Fe16 + AMP. We demonstrated the ability of Fe16 + AMP to disrupt the bacterial membrane of S. aureus and that likely allowed for better absorption of AMP. In addition, the change in gene expression of bacterial efflux pumps at the sub-inhibitory concentration of AMP suggests an insufficient import of iron into the bacterial cell. At the same time, Fe16 + AMP did not have any cytotoxic effects on keratinocytes. Combined Fe16 + AMP therapy demonstrated significant synergistic and antimicrobial effects against S. aureus. This study supports the potential of combination therapy and further research.

Keywords

Staphylococcus aureus; Iron complex; Ampicillin; Synergy; Antimicrobial activity

Authors

KOŠARIŠŤANOVÁ, L.; ŘÍHÁČEK, M.; SUCHÁ, F.; MILOSAVLJEVIĆ, V.; ŠVEC, P.; DORAZILOVÁ, J.; VOJTOVÁ, L.; ANTAL, P.; KOPEL, P.; PATOČKA, Z.; ADAM, V.; ŽŮREK, L.; DOLEŽELÍKOVÁ, K.

Released

6. 10. 2023

Publisher

BMC

Location

LONDON

ISBN

1471-2180

Periodical

BMC MICROBIOLOGY

Year of study

Number

State

United Kingdom of Great Britain and Northern Ireland

Pages from

Pages to

Pages count

URL

https://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-023-03034-1

Full text in the Digital Library

http://hdl.handle.net/11012/245098

BibTex

@article{BUT185666,
  author="Ludmila {Košarišťanová} and Martin {Říháček} and Františka {Suchá} and Vedran {Milosavljević} and Pavel {Švec} and Jana {Matulová} and Lucy {Vojtová} and Peter {Antal} and Pavel {Kopel} and Zdeněk {Patočka} and Vojtěch {Adam} and Luděk {Žůrek} and Kristýna {Doleželíková}",
  title="Synergistic antibacterial action of the iron complex and ampicillin against Staphylococcus aureus",
  journal="BMC MICROBIOLOGY",
  year="2023",
  volume="23",
  number="1",
  pages="1--8",
  doi="10.1186/s12866-023-03034-1",
  issn="1471-2180",
  url="https://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-023-03034-1"
}