Publication detail
Use of Calcium Chloride for Removal of Polysaccharides During DNA Isolation from Tropical Fruit Products
FIALOVÁ, L. ŽYLKOVÁ, K. MÁROVÁ, I.
Original Title
Use of Calcium Chloride for Removal of Polysaccharides During DNA Isolation from Tropical Fruit Products
Type
abstract
Language
English
Original Abstract
Food products made out of certain fruits (such as mango) may be subject to adulteration. The adulteration may be detected by various methods, including PCR-based ones. However, PCR-based methods are susceptible to inhibition if the DNA sample is contaminated with certain polysaccharides. Furthermore, polysaccharide contamination is notoriously difficult to remove. In this work we demonstrate the use of calcium chloride for successful removal of potentially inhibitory polysaccharides from samples which are known to contain them (mango and banana puree). We also address the potential inhibitory effect of calcium chloride itself and we show a way in which polysaccharide precipitation by calcium chloride may be added to a DNA isolation protocol without hampering the amplifiability of the isolated DNA.
Keywords
PCR, DNA isolation, polysaccharides, calcium chloride, mango
Authors
FIALOVÁ, L.; ŽYLKOVÁ, K.; MÁROVÁ, I.
Released
23. 10. 2023
Publisher
Institute of Analytical Chemistry of the CAS, v. v. i., Veveri 97, 602 00 Brno, Czech Republic
Location
Brno
ISBN
978-80-908154-0-7
Book
CECE 2023 18th International Interdisciplinary Meeting on Bioanalysis
Edition
1
Edition number
1
Pages from
50
Pages to
50
Pages count
1
URL
BibTex
@misc{BUT185429,
author="Lenka {Fialová} and Kateřina {Žylková} and Ivana {Márová}",
title="Use of Calcium Chloride for Removal of Polysaccharides During DNA Isolation from
Tropical Fruit Products",
booktitle="CECE 2023 18th International Interdisciplinary Meeting on Bioanalysis",
year="2023",
series="1",
edition="1",
pages="50--50",
publisher="Institute of Analytical Chemistry of the CAS, v. v. i., Veveri 97, 602 00 Brno, Czech Republic",
address="Brno",
isbn="978-80-908154-0-7",
url="https://www.ce-ce.org/user_uploads/CECE_2023/CECE%202023%20Proceedings.pdf",
note="abstract"
}