Publication detail

Advanced Bone Regeneration: 3D Printed Scaffolds Enhanced with Bioactive Proteins

IZSÁK, D. LYSÁKOVÁ, K. MENČÍK, P. KADLECOVÁ, Z. PAVLIŇÁKOVÁ, V. VOJTOVÁ, L.

Original Title

Type

abstract

Language

English

Original Abstract

Extensive bone defects pose a significant research challenge thanks to their slow healing process. The application of 3D printing offers a compelling alternative to traditional methods involving manual processing and casting. This is primarily due to its capacity to define the shape, dimensions, and structure of the product. This revolutionary technique facilitates the precise, and cost-efficient production of bone substitutes for regenerative medicine. In this project, a unique bio-ink was prepared by mixing calcium phosphate ceramic powder (CaP) with a thixotropic biodegradable thermosensitive copolymer and a biocompatible polysaccharide. The main goal was to create a non-toxic and completely bioresorbable material suitable for the human body. This bio-ink exhibits thixotropic characteristics, enabling it to transition into a printable biomaterial suitable for direct ink writing (DIW) technology. The scaffolds were carefully crafted following a biomimetic approach involving 3D printing at lower temperatures. To support the healing process, bioactive proteins with the ability to boost regeneration or suppressing infections were infused into the printed scaffolds. These proteins were incorporated after the printing process using a coating technique. Subsequently, the scaffolds were solidified in a high-humidity environment. The release of proteins was conducted at body temperature within a physiological solution (phosphate buffer saline) and monitored using enzyme-linked immunosorbent assay (ELISA). The structural and morphological characteristics of the printed samples, printed at varying extrusion coefficients, were analyzed using scanning electron microscopy. This comprehensive analysis offered valuable insights into the structural and morphological alterations induced by distinct printing conditions. This work was supported by the ProfiBONE project (TO01000309) that benefits from the EEA Grants and the TACR within the KAPPA Programme. CzechNanoLab project LM2023051 funded by MEYS CR is gratefully acknowledged for the financial support of the measurements at CEITEC Nano Research Infrastructure.

Keywords

3D printing, release, protein coating, extrusion coefficient, morphology, scaffolds, regenerative medicine, bone cement, bio-ink

Authors

IZSÁK, D.; LYSÁKOVÁ, K.; MENČÍK, P.; KADLECOVÁ, Z.; PAVLIŇÁKOVÁ, V.; VOJTOVÁ, L.

Released

26. 10. 2023

Location

Brno

Pages from

Pages to

Pages count

108

URL

https://drive.google.com/file/d/1TSTUdgBvfOhhfbUBjU5ceFjHqkLI3vyo/view?usp=sharing

BibTex

@misc{BUT185044,
  author="Dávid {Izsák} and Klára {Tušlová} and Přemysl {Menčík} and Zuzana {Kadlecová} and Veronika {Pavliňáková} and Lucy {Vojtová}",
  title="Advanced Bone Regeneration: 3D Printed Scaffolds Enhanced with Bioactive Proteins",
  booktitle="Postdoc and PhD RETREAT Organised by CEITEC Postdoc and PhD committees, BOOK OF ABSTRACTS, 26.-27 October 2023, Milovy, Czechia",
  year="2023",
  pages="81--81",
  address="Brno",
  url="https://drive.google.com/file/d/1TSTUdgBvfOhhfbUBjU5ceFjHqkLI3vyo/view?usp=sharing",
  note="abstract"
}